Words similar to aptamer
Example sentences for: aptamer
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In order to examine effector-specificity in vivo , a new series of Group I aptazymes was constructed in which the anti-theophylline aptamer was mutated to bind 3-methylxanthine (3MeX2P6) [ 34 ] . These variants proved to be responsive to 3-methylxanthine both in vitro and in vivo (Figure 6a).
Based on these studies, we designed a series of Group I aptazymes in which the anti-theophylline aptamer was substituted for either a portion of P6 or P5 (Figure 1).
The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.
In particular, a series of nested deletions of the P6 stem-loop have been shown to partially or completely compromise ribozyme activity [ 15 ] . More importantly, either magnesium or the tyrosyl tRNA synthetase from Neurospora mitochondria (CYT18) can suppress many of these defects [ 16 17 ] . Previous studies with other introns have also revealed that deletion of the P5 stem-loop can modulate ribozyme activity [ 18 19 ] . We hypothesized that sites where deletions modulated ribozyme activity might also prove to be sites where aptamer conformational changes would modulate ribozyme activity.
Group I aptazymes that were dependent upon theophylline both in vitro and in vivo were engineered by conjoining the thymidylate synthase ( td ) intron with an anti-theophylline aptamer.
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