Words similar to aptamer
Example sentences for: aptamer
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The effector specificity could be rationally changed by changing the specificity of the conjoined aptamer: when a mutation was introduced into the anti-theophylline aptamer that led to discrimination against theophylline and in favor of 3-methylxanthine (that also differed from theophylline by a single methyl group), the aptamer became solely responsive to 3-methylxanthine in vivo . Group I aptazymes may have a number of important biotechnology applications, including use as in vivo , real-time reporters and as regulatable gene therapeutics.
The ligand specificity of the anti-theophylline aptamer carried over to the theophylline-dependent aptazyme, and no activation was seen with caffeine, which differed by a single methyl group from theophylline.
For example, the junction between the aptamer and the Group I ribozyme in the aptazyme Th2P6 resembled the deletion variant td ΔP6-6; the aptazyme was activated by theophylline, while the splicing defect of the deletion variant at 3 mM magnesium was suppressed by 8 mM magnesium or by stabilization of a tetraloop sequence that capped the P6 helix [ 15 ] . Defects that poise a ribozyme between active and inactive conformers have previously been used to engineer effector-dependence [ 20 ] .
In particular, a series of nested deletions of the P6 stem-loop have been shown to partially or completely compromise ribozyme activity [ 15 ] . More importantly, either magnesium or the tyrosyl tRNA synthetase from Neurospora mitochondria (CYT18) can suppress many of these defects [ 16 17 ] . Previous studies with other introns have also revealed that deletion of the P5 stem-loop can modulate ribozyme activity [ 18 19 ] . We hypothesized that sites where deletions modulated ribozyme activity might also prove to be sites where aptamer conformational changes would modulate ribozyme activity.
Group I aptazymes that were dependent upon theophylline both in vitro and in vivo were engineered by conjoining the thymidylate synthase ( td ) intron with an anti-theophylline aptamer.
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