Words similar to aptazyme

Example sentences for: aptazyme

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Each of the successful aptazyme constructs was subsequently cloned into an interrupted thymidylate synthase gene in place of the parental td self-splicing intron.
Interestingly, those aptazyme constructs that showed ligand-dependent activity closely resembled the original deletion variants that showed magnesium-dependent recovery of splicing activity.
In contrast, the original aptamer was known to also bind 3-methylxanthine, and the original Group I aptazyme (Th2P6) also proved to be responsive to 3-methylxanthine in vivo (Figure 6c).
The parental P6 aptazyme construct was generated by two overlapping oligos, Gp1Wt2 Gp1Wt2.
Finally, no growth was observed in a negative control that contained a non-functional Group I intron (B11), and no theophylline-dependent changes in growth were observed in a negative control in which mutations were introduced into the aptazyme to abolish theophylline binding (Th2P6.D).