Words similar to aptazyme

Example sentences for: aptazyme

How can you use “aptazyme” in a sentence? Here are some example sentences to help you improve your vocabulary:

The aptazyme modified at P5 (Th1P5) showed less theophylline-dependent enhancement of the extent of reaction than the aptazyme modified at P6 (Th2P6), consistent with the single point assays described above.
Similarly, it may prove possible to use effector-dependent splicing to more finely monitor gene expression in vivo . A drug could be localized to particular organs, cells, or organelles, and splicing of the aptazyme could be monitored via a reporter gene such as luciferase.
Interestingly, no growth was observed for bacteria harboring the intron Th1P6, despite the fact that this aptazyme showed the greatest activation by theophylline in vitro . The plating results were consistent and reproducible over a variety of experiments.
These levels of ligand-dependent activation were similar to those observed with the original hammerhead aptazyme constructs, [ 21 ] and it may prove possible to use in vitro selection to further optimize activation [ 13 14 ] . However, when the time course data were fit with a single exponential equation, the rates of the initial reactions turned out to be very similar in both the presence and absence of theophylline (Table 2).
In order to convert the Group I self-splicing intron to a regulatable aptazyme, it was necessary to first identify sequences or structures in the ribozyme whose conformation might modulate splicing activity.