Words similar to annealing
Example sentences for: annealing
How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:
α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.
Amplification reactions included the high fidelity DNA polymerase, Pfu (Stratagene, La Jolla, CA) and an annealing temperature of 58°C.
In at least most cases, weak and null alleles appear to be due to substitution polymorphisms within the primer annealing sites [ 6 ] . They can be repaired by sliding the offending PCR primer to a nearby position along the chromosome.
Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 45 seconds, 63°C for 60 seconds for annealing, and 72°C for 90 seconds for extension.
For example, we would like to know whether using alocal search algorithm, such as back propagation or simulated annealing [ 35 ] , to refine the weights of a GPNN modelis useful.