Words similar to annealing
Example sentences for: annealing
How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:
All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.
PCRs were performed as described above except that 1 μl of pooled BAC library DNA was used as template and the annealing temperature for most markers was raised to 60°C to achieve greater specificity.
The thermal cycling conditions were 40 cycles of the denaturation step at 95°C for 30 seconds, annealing at 60°C for one minute and an extention step at 72°C for one minute.
We conducted the PCR reaction for 36 cycles of denature, annealing and extension (15 s at 94°C, 30 s at 55°C, 30 s at 68°C), followed by a final extension of 4 min at 72°C.
It has been suggested in the case of RecT that it may compete with SSB for binding single strand overhangs and thereby make them available for the annealing process [ 3 ] . Similar interactions between other SSAPs and SSB, that probably coats the ssDNA generated by nucleases, appear likely.