Example sentences for: annealing

How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR parameters were as follows: First cycle of incubation at 95°C for 2 min followed by 35 cycles of incubation at 95°C for 2 min, at annealing temperature for 1 min and at 72°C for 1.5 min.

  • In at least most cases, weak and null alleles appear to be due to substitution polymorphisms within the primer annealing sites [ 6 ] . They can be repaired by sliding the offending PCR primer to a nearby position along the chromosome.

  • PCR conditions other than annealing temperature used were the same as described above.

  • The PCR was allowed to proceed in each case for 30 cycles of 1 minute at 94°C, 1 minute at the annealing temperature, and 1 minute at 72°C.

  • For the MRP gene, after an initial denaturation step of 95°C for 4 min, 35 cycles of PCR amplification were performed, each cycle consisting of a denaturing step of 95°C for 25 s, annealing at 54°C for 30 s, and extension at 73°C for 60 s, followed by a final step at 72°C for 8 min.


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