Words similar to annealing
Example sentences for: annealing
How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:
Gene specific primer sequences, cycle times and annealing temperatures are outlined in Table 1. For each transcript, raw densitometry data was subjected to paired t-testing (two-sided, df = 7) to determine whether differences in expression existed in mefloquine and DMSO-treated cultures ( P < 0.05).
Thermal cycler parameters included 2 min at 50°C, 10 min at 95°C, and 40 cycles involving denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min.
The annealing/ ligation reaction therefore included both of the allele-specific OCPs (OCP-1822C and OCP-1822T, Table I), and the ERCA reaction was performed in the presence of both of the allele-specific P2 Amplifluor primers (P2-1822C-TET and P2-1822T-FAM).
A typical reaction cycle was denaturation for 3 min at 94°C followed by 35 cycles of denaturation for 15 s at 94°C, annealing for 1 min 15 s at 54°C, and extension for 1 min 15 s at 72°C with a final extension step at 72°C for 10 min.
The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1:1, denaturing and annealing.