Example sentences for: annealing

How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5°C/cycle) "touch-down" annealing temperature.

  • The PCR cycling conditions consisted of a denaturation step at 94°C for 30 s, an annealing step at 54°C (S12) or 60°C (TNF-α), an extension step at 72°C for 1 min, and a final extension step at 72°C for 10 min.

  • DNA was denatured by heating the reactions at 95°C for 3 min followed by annealing and ligation at 60°C for 20 min.

  • DNA sequences were determined by reamplifying the original genomic DNA using the appropriate family-specific V H leader and J H consensus primers under the following PCR conditions: denaturation at 94°C for 45s; annealing at 62°C for 30s; and extension at 72°C for 45s.

  • All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.


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