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Example sentences for: annealing

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The annealing temperatures for each primer pair are listed in Table 1. Sequencing was with dye-labeled terminators (ABI 377 Sequencer).
All components except Ampligase were pre-warmed to the reaction temperature (40, 47, 55, or 63°C) for 20 min to allow OCP annealing to come to equilibrium.
Cycling parameters were an initial denaturation of 94°C for 4 min, followed by 30 cycles of denaturation at 94°C for 1 min, annealing for 45 sec and extension at 72°C for 1 min 30 sec, followed by a final extension of 72°C for 7 min.
2 (reverse 5'-CAGATGAGACTGGGAAAGGC-3') at an annealing temperature of 55 degrees Celsius for 35 cycles, was designed to the CHRNA7 -related portion of BAC RP11-40J8 (accession AC010799).
The PCR programs for most primer pairs were as follows: 94°C/3 min for initial denaturation; 94°C/30 sec, 65 to 56°C/30 sec, 72°C/1 min, for two cycles at each annealing temperature; 94°C/15 sec, 55°C/30 sec, 72°C/1 min for 20 cycles; and 72°C/10 min final extension [ 23 ] . For some primer pairs, such as PM36, PM 42, PM45, PM50, PM53, and PM60, the PCR conditions should be optimized to find suitable annealing temperature, such as 35 cycles of 94°C/30 sec, 60°C/1 min or 50°C/1 min, 72°C/1 min (see Table 2).