Example sentences for: annealing

How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.

  • Amplification reactions included the high fidelity DNA polymerase, Pfu (Stratagene, La Jolla, CA) and an annealing temperature of 58°C.

  • In at least most cases, weak and null alleles appear to be due to substitution polymorphisms within the primer annealing sites [ 6 ] . They can be repaired by sliding the offending PCR primer to a nearby position along the chromosome.

  • Thirty-five cycles of the polymerase chain reaction utilizing a microprocessor controlled thermal cycler (Perkin-Elmer, Norwalk, CT) were then performed to amplify the desired segment utilizing the following parameters; 94°C for denaturation for 45 seconds, 63°C for 60 seconds for annealing, and 72°C for 90 seconds for extension.

  • For example, we would like to know whether using alocal search algorithm, such as back propagation or simulated annealing [ 35 ] , to refine the weights of a GPNN modelis useful.


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