Example sentences for: annealing

How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR primers used for GAPDH and calbindin D were as previously described [ 22 ] . PCR conditions used were: denature at 94°C for 40 s, annealing at 55°C for 50 s with an extension at 72°C for 50 s.

  • All real-time PCR reactions were performed in triplicate on an ABI Prism 7000 in SYBR Green Master Mix (Applied Biosystems), with denaturation at 94°C for 15 seconds, annealing at 55°C for one minute and extension at 60°C for one minute in a total of 40 cycles.

  • 2 (reverse 5'-CAGATGAGACTGGGAAAGGC-3') at an annealing temperature of 55 degrees Celsius for 35 cycles, was designed to the CHRNA7 -related portion of BAC RP11-40J8 (accession AC010799).

  • Cycles (n = 40) consisted of a 15 second melt at 95°C, followed by a 1 minute annealing/extension at 60°C.

  • PCR conditions were optimized and linear amplification range was determined for each primer by varying annealing temperature and cycle number.


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