Example sentences for: annealing

How can you use “annealing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In case of the DAMD PCR, annealing temperature of 50°C was found to be optimum for generating distinct profiles with all three primers.

  • The similarity between these two systems is further extended by the observation that the RecT/E system can complement mutations in the λ exo/Redβ system [ 10 12 ] . In eukaryotes, RAD52 protein has been shown to exhibit properties similar to those of RecT and Redβ proteins: it binds ssDNA and promotes strand exchange via the pairing of complementary single strands [ 6 13 ] . In vitro studies on quaternary structures have shown that the single strand annealing proteins (SSAPs), RecT, Redβ, ERF and RAD52, form similar helical super-structures [ 14 15 16 17 ] . This has led to the proposal that RecT, Redβ, ERF and the eukaryotic RAD52 function in an analogous fashion, and even are "structural homologs" [ 14 ] .

  • CASP4 demonstrated further improvement in our methodologies in both comparative modelling and ab initio prediction [ 26 ] . Our approaches combine a Monte Carlo procedure, simulated annealing, genetic algorithms, graph theory, and semi-exhaustive searches with move sets consisting of fragments and discrete state models, and scoring functions consisting of all atom-based pairwise functions, hydrophobicity indices, secondary structure preferences, and hydrogen bonding.

  • A blunt-end linker oligomer was designed according to Mueller and Wold [ 55 ] , and was formed by annealing a mixture of oligomers 7A and 7B, 20 μm each, in DNAb/50, by first heating in a 100° temperature block for 1 minute, and then allowing the block to cool to room temperature, sitting on the bench top.

  • The thermal profile for long-range PCR was as follows: denaturation for 2 min at 94°C, then 35 cycles of denaturation (10 s at 94°C), annealing (30 s at 60°C), and extension (8 min at 68°C), followed by a final extension step of 7 min at 68°C.


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