Example sentences for: annealed

How can you use “annealed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The amplified annealed fragment was gel purified and cloned into the Sma I site of pUC19 to generate pSE.

  • An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.

  • The negative control luciferase RNA was the same as the double-stranded GL3 RNA described previously [ 17 ] . The RNAs were annealed in vitro to form double-stranded RNA (dsRNA) [ 18 ] and electroporation was carried out essentially as described [ 32 ] . In brief, 3 μg dsRNA in 800 μl of incomplete cytomix [ 32 ] was added to infected RBC (at 10-15% parasitemia), and electroporation was performed using a Bio-Rad Gene Pulsar unit at settings of 200 Ω, 2 kV, and 25 μF.

  • The T m for the 3' arm of the probe annealed to target was designed to be between 60-65°C.

  • 7B) were synthesized (~50 bases in length) and subsequently annealed to get double stranded oligos.


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