Example sentences for: annealed

How can you use “annealed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 4 nM) annealed to 5'- 32P-labeled 21-mer primer (0.

  • The rationale for pre-extension [ 8 ] was that cut template molecules that are annealed to reference molecules should be extended by the polymerase to become full length.

  • The FADI primer was annealed at 48°C and the PLYT primer was annealed at 20°C.

  • One OCP annealed perfectly to the normal sequence of interest, and the other targeted the mutant.

  • The negative control luciferase RNA was the same as the double-stranded GL3 RNA described previously [ 17 ] . The RNAs were annealed in vitro to form double-stranded RNA (dsRNA) [ 18 ] and electroporation was carried out essentially as described [ 32 ] . In brief, 3 μg dsRNA in 800 μl of incomplete cytomix [ 32 ] was added to infected RBC (at 10-15% parasitemia), and electroporation was performed using a Bio-Rad Gene Pulsar unit at settings of 200 Ω, 2 kV, and 25 μF.


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