Example sentences for: annealed

How can you use “annealed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The amplified annealed fragment was gel purified and cloned into the Sma I site of pUC19 to generate pSE.

  • 200-400 ng of whole-cell DNA was denatured and annealed with an "Extend" primer using Vent (exo-) polymerase (New England BioLabs).

  • Because the micrococcal nuclease-treated cores may represent the best likelihood of observing RNAseH activity, a titration of the substrate was performed employing 50 to 500 ng DXB+ RNA annealed to D1452- and nuclease-treated DHBV core particles.

  • The C-terminal dabcyl was positioned so that it was proximal to the 5'-fluorescent moiety on the DNA reporter, quenching fluorescence when the reporter and Q-PNA-13 were annealed, as shown in Figure 2. After P1 primer is incorporated into ERCA product, Q-PNA is displaced, producing a fluorescent signal.

  • an appropriate nested primer is 5'- 32P-labeled as previously described [ 54 ] ; 5 μl of the P1+7A reaction or the step-in verification reaction is denatured and annealed with 1 pmol of the end-labeled display primer and 0.5 units of Taq polymerase (Qiagen) in 10 μl final volume of 1× "Taq salts" (Qiagen).

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