Example sentences for: annealed

How can you use “annealed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To determine if the lack of success was specific to the DRF+ RNA, a second RNA, DXB+ (DHBV nt 1358-1504 positive polarity), annealed to the complementary oligonucleotide D1452- was employed in RNAseH assays.

  • This adapter is annealed to the genomic DNA fragments, and Klenow is added for second strand synthesis.

  • An in vitro assay using dideoxy terminated primer annealed with the template which allows the next correct dNTP to bind in the ternary complex without actual DNA synthesis has recently been reported [ 25 ] . Using this assay system, we have evaluated the ability of the insertion mutants to form the ternary complexes and the effect of DNA trap on such complexes.

  • OCPs were designed such that the 3' terminal nucleotide annealed opposite the mutation of interest (Figure 1).

  • The T m for the 3' arm of the probe annealed to target was designed to be between 60-65°C.


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