Words similar to alu
Example sentences for: alu
How can you use “alu” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 U Bfa I, 1.0 U Dde I, 1.5 U Dpn I, 2.0 U Alu I, 1.0 U Rsa I, or 2.0 U Hinf I) were diluted in 10 μl buffer (20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA) and added separately to the six rections (FAM: Bfa I and Dde I, JOE: Dpn I and Alu I, NED: Rsa I and Hinf I).
This result is completely consistent with previous studies of the L1 endonuclease targeting preference in vitro and as inferred from the TSDs of Alu elements, which are thought to hijack the L1 machinery for their insertion [ 6, 8, 53].
The radial cloning method involves isolation of a set of nested overlapping fragments that extend from the gene-specific targeting hook to upstream or downstream Alu (or B1 ) positions in the gene of interest.
Not only does L1 sequence itself contribute at least 462 megabases (Mb) to our genome (17% of the total length) [ 1 ] , but copies of the Alu and SVA transposable elements and processed pseudogenes are also believed to have inserted into the genome by borrowing the EN and RT proteins encoded by L1 [ 13 20 21 24 25 26 27 ] . In addition to self-mobilization and mobilization of other transposable elements, L1s can also move unique flanking DNA sequence to another locus in the genome in a process known as 3' transduction.
The radiolabeled PCR products were digested with Alu I or Hpa II, denatured and electrophoresed for 10-16 h at 500 V on non-denaturing 6% acrylamide gels at room temperature [ 24 ] . For RFLP analysis, PCR products were digested with Mbo II and analyzed by electrophoresis on non-denaturing 6% acrylamide gels at 500 V for 3-4 hours.
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