Words similar to alanyl

Example sentences for: alanyl

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Streptococcus mutans in which DCP is inactive do not initiate growth at below pH 6.5 and make glycerol LTA without D-alanine [ 14 ] . In the DCP active strains, soluble D-Alanyl LTA is extruded into culture fluid in vitro [ 16 17 ] or plaque in vivo [ 18 ] . The D-alanyl esters are stable at pH 6.0 at 37°C, but hydrolyze to free D-alanine and LTA with a half-life of 3.9 h at pH 8.0 [ 19 ] . Healthy gingival sulci have a pH of 6.5 - 7.5 and inflamed sulci a pH of 7.5-8.
D-alanyl LTA, but not D-alanine-free LTA, is present in culture filtrates of Streptococcus mutans GS5 [ 17 ] . Bacteria were grown at 37°C in trypticase soy broth to late stationary phase (96 h), when the maximal amount of antigen is extruded into the culture fluid [ 18 ] . After centrifugation to remove the bacteria, culture fluid (10 1) was concentrated 20-fold over a YM10 Diaflo Membrane filter (Amicon Corp., Beverley, MA).
The rationale behind this study was that acidic plaque environments increase the amount of D-alanyl LTA and promote its immunogenicity.
About 30% of young adults have serum IgG antibodies that precipitate with D-alanyl LTA, but not with D-alanine-free LTA [ 17 21 ] . It is likely that plaques induce these IgG antibodies from gingival sulci that contain more acid-tolerant bacteria.
DMFT associated with age, as reported for other subjects whose sera did not precipitate D-alanyl LTA [ 22 ] , and also with pocket depth.