Words similar to agc
Example sentences for: agc
How can you use “agc” in a sentence? Here are some example sentences to help you improve your vocabulary:
For rabbit EP2: 5'-TCC TCC CGA AAA GAA AAG TGG-3' for sense and 5'-TGT TTA CCC CGT TTT ATC AGG-3' for antisense; for rabbit GAPDH: 5'-CGG AGC CAA AAG GGT CAT CAT-3' for sense and 5'-TTT CTC CAG GCG GCA GGT CAG-3' for antisense.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
It had previously been reported that the invariant G at position 2 of the catalytic AGC triad in domain V was critical for self splicing in vitro and in vivo . Here, point mutants of this residue were made.
The primers (5' or 3') used for COX-2 were sense primer, CAT TCT TTG CCC AGC ACT TCA C and antisense primer, GAC CAG GCA CCA AGA CCA AAG AC.
A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
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