Words similar to adapter
Example sentences for: adapter
How can you use “adapter” in a sentence? Here are some example sentences to help you improve your vocabulary:
CT adapter: AAC TGC AGG AGG GAC AGT TGA AGG AGG CA CC TCC GTG C
The Taq I fragments of HL60 cDNA were ligated with the CT adapter at 16°C overnight with 3 μl (9 units) of T4 ligase (Promega, Madison, WI) while the Taq I fragments of HL60/TLK286 cDNA were ligated with the TT adapter.
Since K430 is located on the smaller, NH 2 -terminal lobe of the kinase domain, whereas R525 is part of the larger, COOH-terminal lobe, it is possible that the two mutations differentially affect the capacity of kinase inactive Btk to function as an adapter in the activation of PLCγ2.
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
The deletion alleles of BLM were constructed using digestion with two restriction enzymes and insertion of an oligonucleotide adapter.
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