Words similar to adapter
Example sentences for: adapter
How can you use “adapter” in a sentence? Here are some example sentences to help you improve your vocabulary:
Upon BCR stimulation, Btk is thought to be recruited to PLCγ2 via the adapter protein BLNK, as BLNK can associate with the SH2 domains of both PLCγ2 [ 31, 32] and Btk [ 33].
Baugh and colleagues were able to improve yields when amplifying mRNA by decreasing the concentration of T7 primer [ 16 ] . When we limited the mass of T7 primer adapter to approximately five times that of the starting material with a lower limit of 50 ng (by decreasing the second strand synthesis reaction volume), this low molecular weight species disappeared, without decreasing overall yields (data not shown).
In addition to extending the primer adapter, the 3'-5' exonuclease activity of Klenow should trim 3' polyTs in excess of the 18 bps complementary to the adapter, and allow fill in of the appropriate complementary bases (Fig 1).
EcoR I adapter was added, the cDNA was digested by Xho I and the cDNA was inserted between the EcoR I and Xho I sites of the vectors in a directional fashion.
Finally, the RNAs are reverse transcribed followed by PCR amplification using a gene-specific primer and a primer homologous to the RNA oligonucleotide adapter.