Words similar to adaptamer
Example sentences for: adaptamer
How can you use “adaptamer” in a sentence? Here are some example sentences to help you improve your vocabulary:
The adaptamer sequences become the basis for re-amplification of the gene sequences in a second stage amplification.
During the second stage of amplification, the first stage PCR products serve as template, whereas a single pair of primers recognizing the flanking adaptamer sequence can be used to re-amplify all products of the first stage PCR.
The use of adaptamer sequences with identical T m 's for the 2 ndround of amplification optimized the yields, despite different product lengths and different template concentrations (which were dependent on the yield from the first round reactions).
Apparently, the amount of correct template produced by the 1 stround PCR, albeit often invisible during gel analysis, was usually sufficient to give high yields during the 2 ndround of amplification using the common adaptamer sequences.
Importantly, the two-stage amplification had the unanticipated advantage of normalizing the yield of PCR products across the entire set of genes, such that lower than average yielding products from the 1 stround PCR (using the gene specific sequence and chromosomal DNA as template) often had a robust yield during the 2 ndround of amplification using the common adaptamer sequences.