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Example sentences for: acsf

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reported that under epileptogenic condition (free Mg 2+in ACSF), γ band oscillations arise from GABAergic depolarizations and that this activity may lead to the generation of ictal discharges [ 18 ] . It has been reported that prolonged periods of γ oscillations are associated with temporal lobe seizures in in vivo rats [ 41 ] . Furthermore, human EEG studies with subdural recording electodes showed that a γ band oscillation could be recorded at the start of typical seizure activity [ 42 ] . In this respect, the hyperthermic γ oscillations may also play a critical role in generation of neuronal epileptiform activity.
In the same slice, tetanic stimulation delivered in control artificial cerebrospinal fluid (ACSF) induced typical γ oscillations, and these γ oscillations were abolished by addition of BMI to the perfusate.
γ oscillations recovered partially by 80 minutes of wash in ACSF.
Slices were allowed to incubate and recover for at least an hour in room temperature ACSF comprised of the following composition (mM): NaCl 117; KCl 5.4; NaHCO 3 26; MgSO 4 1.3; NaH 2 PO 4 1.2; CaCl 2 2.5; glucose 10, continuously bubbled with 95% O 2 plus 5% CO 2 .
The brain tissue was removed rapidly and placed in iced artificial cerebrospinal fluid (ACSF).