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Example sentences for: acids
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Net1N includes the N-terminal 341 amino acids of Net1 and is sufficient to bind and inhibit Cdc14 [ 11 ] . When GST-Cdc14/His6-T7-Net1N complex anchored to anti-T7 beads was treated with ATP plus increasing amounts of recombinant Cdc5 purified from insect cells, both Cdc14 and Net1N were phosphorylated as indicated by their increased apparent molecular weight.
The output from each search is further analyzed based on criteria described in Table 1. Homology between the partner and surrogate oftentimes ranges over a long stretch (15-20 amino acids) or may be found in a perfect match within a short sequence of 5-8 amino acids (unpublished data).
In order to identify the interaction of amino acids in this binding loop with 5-HT 3 R ligands, we have constructed alanine mutations of residues throughout this region and evaluated the alteration in binding affinity of 5 different classes of 5-HT 3 R ligands (Figure 1).
Indeed, both human homologs of yPOP2, hCAF1 and hPOP2, lack the 148 N-terminal amino acids which are, in yPOP2, required for transcriptional activation, whereas the yPOP2 C-terminus, which is involved in the interaction with yCCR4, is conserved in hCAF1 [ 11 ] and hPOP2 [ 19 ] . Similarly, hNOT2, the human homolog of the yNOT2 component of the CCR4-NOT complex, harbors a N-terminal domain divergent from that of yNOT2 -required for transcriptional activation and interaction with the yeast ADA2 factor-, but has conserved the C-terminal domain which is absolutely required for the yCCR4-associated function of yNOT2 [ 19 20 ] . Therefore, it looks as if genes of the CCR4-NOT complex have undergone a "concerted" phylogenetic evolution, with the yeast genes encoding multifunctional proteins and the animal genes only encoding "specialized" proteins specific for the CCR4-NOT complex.
We used both alanine-scanning and a 'radical' site-directed mutagenesis strategy to identify amino acids critical for the interaction within this region of LcrG.
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