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Example sentences for: abolished
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The identity of these bands was not determined, but we have previously shown that the catalytic subunit of human PP2A, which has a similar size, binds to P [ 22 ] . The specific affinity of all of these proteins for the P protein in the column was ascertained by the demonstration that pre-incubation of the A549 extract with excess purified P abolished their binding to the column.
After a campaign of great destruction, the authorities assured them that slavery would be abolished.
The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.
As shown in the additional file 10, combined treatment with α V and β 6 blocking antibodies almost completely abolished the effect of mature TGFβ 1 on the growth of Panc-1 cells.
And, having already vilified the Republicans as enemies of learning for their proposed cuts in federal education outlays, Clinton and the Democrats would appear hypocritical if they abolished the Department of Education.