Words similar to aaa
Example sentences for: aaa
How can you use “aaa” in a sentence? Here are some example sentences to help you improve your vocabulary:
However, insufficient information exists to relate this last common ancestor to any particular other branch of the presently existing AAA family.
To sequence the junction fragments from both strands, we performed PCR and sequencing with primers 774F and reverse primer E43+355 (5'-CAA AGT CTC TGT CTG GAA GAG AAA TGA TTT TGA-3') for FB774, and with forward primer E44 -343 (5'-CCT TAG CTT CCC AAA GTG CTA GGA TTA-3') and 890R for FB890.
These critical residues lie close to the γ-phosphate site in other AAA proteins with known structures and they are believed to trigger a change in the angle between the N- and C-subdomains of the protomer upon binding of ATP or dissociation of the γ-phosphate [ 18 ] . Indirect evidence suggests that such a conformational change in one protomer can be communicated to the adjacent protomers and result in a cooperative alteration in the shape of the overall hexameric ring [ 19 ] .
A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
The AAA domain of midasin is evolutionarily related to that of dynein, but it appears to lack a microtubule-binding site.
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