Example sentences for: pdh

How can you use “pdh” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • This was then amplified by 35 to 50 cycles of the polymerase chain reaction (PCR, GeneAmp RNA PCR kit, N808-0143, Perkin Elmer, Foster City, CA), with primers (Table 1) designed for osteocalcin, type I collagen α1, type II collagen, BMP-2, BMP-4, type IA BMP receptor, 18S ribosomal RNA, or G3PDH (Cat.

  • A normalized value was obtained by dividing the target cDNA amount by the g3pdh reference.

  • Figure 1shows that the G3PDH fragment is barely detectable even after 30 cycles of amplification in the subtracted sample, while it is clearly detectable in the unsubtracted sample after 20 cycles.

  • The amplimers were UV cross-linked and hybridized with Rapid-Hyb buffer (Amersham) at 42°C using γ- 32P ATP end-labeled oligonucleotides (Table 1, G3PDH probe from Clontech) as probes.

  • From each animal, quantitative real-time PCR determinations of 5 target genes ( cry1,2, per1,2 , and dbp ) and a reference cDNA (glyceraldehyde-3-phosphate dehydrogenase, g3pdh ) were made from the cortex, basal forebrain, and hypothalamus (primer/probe sequences available upon request).


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