Words similar to ionophore-clamped

Example sentences for: ionophore-clamped

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Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] . The validation of lysosomal pH measurements was performed by the calibration curve using ionophore-clamped cells as shown in Figure 3. The ratio of emission fluorescence under excitation at 485 nm versus 440 nm (i.e.
Imaging analysis to calculate the ratio of green fluorescence in lysosomes under excitation by 485 and 440 nm (" [F 535 (λ 485 )/F 535 (λ 440 )]") was carried out as previously described [ 3 ] . The ratiometric data were converted to pH using a calibration curve from the lysosomes of ionophore-clamped cells.