Words similar to zymolyase
Example sentences for: zymolyase
How can you use “zymolyase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were pelleted, resuspended in 4 pellet volumes of buffer S, 1 mM PMSF, Zymolyase (100 ug/ml) and incubated at 30°C for 40 min.
Since the background fluorescence in live, cycling cells was too high to derive conclusions about Rad26-GFP localization (data not shown), we prepared cells using a technique previously described in a study of fission yeast chromatin binding proteins [ 42 ] . An asynchronous population of cycling cells was first permeabilized using Zymolyase (Seikagaku Corp., Tokyo), then extracted with 1% Triton X-100 and finally fixed with methanol.
Cell walls were digested for 20-30 min at 29°C with 50 μg/ml Zymolyase 100 T in the same buffer with the addition of 2 μl/ml β-mercaptoethanol.
5 mg/ml, Sigma) and Zymolyase (0.