Words similar to zymed

Example sentences for: zymed

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Collagen-specific IgG isotypes were detected with peroxidase-labeled rabbit anti-mouse IgG 1 or IgG 2a (Zymed Laboratories, San Francisco, CA, USA).
1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.
Following washes, secondary antibody linked to horseradish peroxidase [anti-rabbit (1:2000, Zymed Laboratories, S. San Francisco, CA) or anti-mouse (1:7500, Amersham, Arlington Heights IL)] was added for one hour and incubated with blots at 4°C.
To quantitate differences in activated (phosphorylated) p53 protein, 3 SN50 and control E15 + 2 explants were sectioned, preincubated with unlabeled goat-anti mouse IgG as previously described [ 9 ] and sequentially incubated with a monoclonal anti-phosphorylated p53 (Ser15) antibody (Cell Signaling Technology, Beverly, MA), biotin-labeled goat anti-mouse IgG, and HRP-labeled SA (Zymed Laboratories, South San Francisco, CA), and counterstained with hematoxylin.
Anti-PHAS1 (4EBP-1) polyclonal antibody was purchased from Zymed.