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Example sentences for: zymed

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Horseradish peroxidase (HRP) labeled streptavidin was from Zymed, South San Francisco, CA.
1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.
We used histochemical staining with Hematoxylin and Eosin to ascertain cell morphology; and Alcian Blue at pH 1 [ 43 ] to detect cartilage matrix sulfated glucosaminoglycans as described before [ 1 ] . The production of Collagen type II was assessed by immunohistochemistry using a monoclonal antibody against mouse type II Collagen (Chemicon), and secondary antibody and colorimetric reaction kit (Histostain Plus, Zymed).
Rabbit anti-mouse CXCR3 antibody was purchased from Zymed Laboratories Inc. (So.
E15 + 2 CONT or SN50-treated explants were sectioned, incubated with anti-PCNA using the Zymed mouse PCNA kit (South San Francisco, CA), and counterstained with hematoxylin as previously described [ 13 ] . In this set of experiments, the cytoplasm appears blue and PCNA-positive cells appear dark brown.