Example sentences for: zymed

How can you use “zymed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Anti-PHAS1 (4EBP-1) polyclonal antibody was purchased from Zymed.

  • E15 + 2 CONT or SN50-treated explants were sectioned, incubated with anti-PCNA using the Zymed mouse PCNA kit (South San Francisco, CA), and counterstained with hematoxylin as previously described [ 13 ] . In this set of experiments, the cytoplasm appears blue and PCNA-positive cells appear dark brown.

  • 5% Tween 20, 1% milk) and incubated with primary antibody for 45 min at 37°C (1:1500 anti-eNOS mono [BD Transduction Laboratories, San Diego, CA, USA]; 1:1500 anti-nNOS poly [Zymed Laboratories, South San Francisco, CA, USA]; 1:200 anti-iNOS poly [Affinity Bioreagents, Golden, CO, USA]; and 1:1500 anti-β-actin mono [Sigma A5441; Sigma-Aldrich, St Louis, MO, USA]).

  • Staining of sections for BrdU incorporation was done using the Zymed BrdU staining kit with Horseradish Peroxidase, and development with DAB for 5 minutes.

  • 1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.


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