Example sentences for: zncl

How can you use “zncl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The inclusion of 100 μM ZnCl 2 in the assay buffer resulted in slightly lower values of K m and higher values for k cat [ 36 ] . The inclusion of higher concentrations of Zn(II) did not further affect the steady-state kinetic constants.

  • Steady-state kinetic studies were conducted with both mutants in buffer containing 100 μM ZnCl 2 to ensure that both Zn(II) binding sites were saturated in these studies.

  • 06 M Tris-HCl, pH 7.5, containing 6 mM CaCl 2 and 1 μM ZnCl) for 18 hours at 37°C.

  • Steady-state kinetic assays were conducted at 25°C in 50 mM cacodylate buffer, pH 7.0, containing 100 μM ZnCl 2 on a HP 5480A diode array UV-Vis spectrophotometer at 25°C.

  • The S224K mutant exhibited a 50% reduction in metal binding (Table 2), and we postulate this is due to electrostatic repulsions between the newly introduced Lys with Zn 2 . In spite of the mutants binding significant amounts of Zn(II), we included 100 μM ZnCl 2 in all of the kinetic buffers to ensure saturation of the metal binding sites and to facilitate direct comparison of the kinetic data.


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