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Example sentences for: zn
How can you use “zn” in a sentence? Here are some example sentences to help you improve your vocabulary:
It also has high affinity for binding to single-stranded DNA on its own when separated from the three tetracysteine motifs [ 36 ] . Based on the structural and DNA-binding properties of the E. coli topoisomerase I 14 kDa domain, one can conclude that it is possible for a subdomain in topoisomerase I to lose the Zn(II) binding cysteines during evolution and still maintains the Zn-ribbon structure and single-strand DNA binding properties [ 7 ] .
The three tetracysteine motifs do not form a stably folded structure on its own, but when combined with the 14 kDa C-terminal domain, forms a stably folded 268 amino acid DNA binding domain (ZD domain) that has higher affinity for single-stranded DNA than the 121 amino acid 14 kDa C-terminal region by itself [ 6 ] . Recent sequence and structural analysis suggests that the 14 kDa domain is evolutionarily related to the three tetracysteine motifs and belongs to the zinc ribbon family [ 7 ] . The ZD domain in E. coli topoisomerase I probably evolved from a domain that binds five Zn(II) originally.
Large-scale over-expression and purification of the mutants resulted in comparable quantities of isolatable enzymes, which had identical CD spectra as wild-type L1 and bound slightly less Zn(II) than wild-type L1 (Table 2).
The number of potential Zn(II) binding cysteine motifs range from none in S. cerevisiae DNA topoisomerase III to four highly conserved tetracysteine motifs in the beta family of the topoisomerase III enzymes [ 38 ] . The Zn(II) domain formed by these tetracysteine motifs may be required for interaction with single-strand DNA in removal of hypernegative supercoils [ 39 ] or disruption of early recombination intermediates between inappropriately paired DNA molecules [ 40 ] .
It has previously been observed that a mutation in the Zn(II) binding motif can affect the cleavage site selectivity of topoisomerase I [ 32 ] even though Top67 by itself can recognize both the cytosine in the -4 position and the junction of single- and double-stranded DNA.
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