Words similar to zn
Example sentences for: zn
How can you use “zn” in a sentence? Here are some example sentences to help you improve your vocabulary:
Steady-state kinetic studies were conducted with both mutants in buffer containing 100 μM ZnCl 2 to ensure that both Zn(II) binding sites were saturated in these studies.
856 nm is the most intense for zinc and was used to determine the Zn content in the samples.
The hyperthermophilic topoisomerase I from Thermotoga maritima has been shown to coordinate one Zn(II) with a unique tetracysteine motif Cys-X-Cys-X 16 -Cys-X-Cys but Zn(II) binding is not required for relaxation activity [ 33 ] . The sequence of this unique tetracysteine motifs is somewhat different from those present in other type IA topoisomerases in that the other tetracysteine motifs always had at least two amino acids separating the pairs of cysteines (Cys-X 2-11 -Cys), instead of just one amino acid (Cys-X-Cys) in T. maritima topoisomerase I [ 33 ] . Therefore the structure and function of the single Zn(II) binding motif in T. maritima may differ from the multiple Zn(II) binding motifs in E. coli topoisomerase I. Direct interaction between DNA and the T. maritima Zn(II) binding motif has not been demonstrated.
Metal analyses of the N233L and N233D mutants showed that both bind nearly two Zn(II) ions (Table 2), like wild-type L1 [ 36 ] . CD spectra of the mutants were similar to those of wild-type L1.
The number of potential Zn(II) binding cysteine motifs range from none in S. cerevisiae DNA topoisomerase III to four highly conserved tetracysteine motifs in the beta family of the topoisomerase III enzymes [ 38 ] . The Zn(II) domain formed by these tetracysteine motifs may be required for interaction with single-strand DNA in removal of hypernegative supercoils [ 39 ] or disruption of early recombination intermediates between inappropriately paired DNA molecules [ 40 ] .
Loading...