Example sentences for: ymut
How can you use “ymut” in a sentence? Here are some example sentences to help you improve your vocabulary:
The mutagensis primers were as follows: for mutC, 5' GCTCGACCT CCA TTC TAA TG T GGG CAG GTG GTT TCA CAG 3'; mutK, 5' CCA TTC TAA TTG GGG C TT TTT GTT TCA CAG GGA GGA AGT 3'; mutE, 5' TTT CAC AGG GA C CTT GTT GAC AGT TCA ACT TCA AAC 3'; Xmut1, 5' CAC AGG GAG GAA GTT CTG TCA TCA ACT TCA AAC AGG 3'; Xmut2, 5' GAG GAA GTT GAC AGT CTA AGA CT A AAC AGG GTT GGC AGT 3'; Xmut3, 5' TGA CAG TTC AAC TTC GTT AGT GGT TGG CAG TGA CAC 3'; Ymut1, 5' TGG CAG TGA CAC AGG TGT ACT CTG CCT GCT TCC CCT 3'; and Ymut2, 5' ACA CAG GCC CAC ACT G AT GCG TTC CCC TTT CCA CCC CTG 3'.
For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.
However, the Ymut1 site contains a very weak homology to the TdT/ML intiator consensus sequence, 5'-PyPyCAPyPyPyPyPy-3' [ 39].
The Xmut2 mutation had a measurable effect (2-fold decrease) on the full level of transcription and the Ymut2 mutation reduced the reporter activity by more than three-fold (figure 5c).
The Ymut2 site, which is the most important site for controlling basal expression, overlaps a potential initiator site fitting the consensus (5' CTT CCC TTT TCC 3') for the ribosomal protein Inr family with only one mismatched base [ 40].
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