Example sentences for: ymut

How can you use “ymut” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For the HincII B plasmid, Xmut and Ymut mutagenesis primers were used in conjunction with GL2 for PCR amplification.

  • The mutagensis primers were as follows: for mutC, 5' GCTCGACCT CCA TTC TAA TG T GGG CAG GTG GTT TCA CAG 3'; mutK, 5' CCA TTC TAA TTG GGG C TT TTT GTT TCA CAG GGA GGA AGT 3'; mutE, 5' TTT CAC AGG GA C CTT GTT GAC AGT TCA ACT TCA AAC 3'; Xmut1, 5' CAC AGG GAG GAA GTT CTG TCA TCA ACT TCA AAC AGG 3'; Xmut2, 5' GAG GAA GTT GAC AGT CTA AGA CT A AAC AGG GTT GGC AGT 3'; Xmut3, 5' TGA CAG TTC AAC TTC GTT AGT GGT TGG CAG TGA CAC 3'; Ymut1, 5' TGG CAG TGA CAC AGG TGT ACT CTG CCT GCT TCC CCT 3'; and Ymut2, 5' ACA CAG GCC CAC ACT G AT GCG TTC CCC TTT CCA CCC CTG 3'.

  • However, the Ymut1 mutation decreased the luciferase activity more than five fold, and brought the transcription activity to slightly below basal levels of CD5 transcription (figure 5c).

  • The mutC, mutK, mutE, Xmut, and Ymut mutagenesis primers were first PCR amplified with GL2 primer (5' CTT TAT GTT TTT GGC GTC TTC CA 3') using StuI B plasmid as template to generate megaprimers.

  • The Ymut2 site, which is the most important site for controlling basal expression, overlaps a potential initiator site fitting the consensus (5' CTT CCC TTT TCC 3') for the ribosomal protein Inr family with only one mismatched base [ 40].


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