Words similar to ycpif

Example sentences for: ycpif

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The resulting plasmid, YCpIF16-PPT1, was transformed into yeast W303 cells ( MAT a , can1-100, leu2-3,112, his3-11,15, trp1-1, ura3-1, ade2-1 ). Transformed cells were grown in SC-Trp medium at 30°C to OD 600 nm 0.5, washed twice to remove glucose, then grown in SG-Trp medium containing 2% galactose instead of glucose for 12-16 hr at room temperature to induce protein expression.
GAL1 promoter, a PPT1 DNA fragment was cut from pET-GST-PPT1 by EcoR I and Not I digestion and subcloned into the EcoR I/ Not I-digested YCpIF16 (ATCC No.
In the 3' primer, the sequence from the Xho I restriction site to the HA coding sequence was identical to that present in YCpIF16.
Then, a fragment containing the PPT1 ORF was excised from YCpIF16-PPT1 by Xho I and Not I digestion and subcloned into this plasmid to form pRS306-955HAPPT1.