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Example sentences for: yacs

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Chromosome-size DNA was prepared from yeast transformants carrying circular YACs, separated by clamped homogeneous electrical field gel electrophoresis (CHEF), transferred onto a nylon membrane, and hybridized with an alphoid DNA probe or Alu -probe (see above).
The size of the YACs varied from 50 kb to 210 kb (Fig.
Initially, to determine whether sarcospan was contained on YACs or BACs spanning the CFEOM1 critical region, YACs 766h7, 762e1, 951h6, 813h9, 782e8, 813g11, 832f4, 887g10, 916c8, 936f5, 946d5, 852c3, 723h3, 906f9, 798b12, 958b2, 943f6, 957b8, and 973h3 [ 2] and BACs B455J16, B50I19, B459A22, B392F18, B193D8, B855O3, B460N10, B351C12, B56H16, B937H2, B1035D8, B251K10, B152M7, B396F22, B45D10, B471G7, B520I18, and B367O10 (AECOM Genome Center Home Page chromosome 12 maps http://sequence.aecom.yu.edu/chr12/QARM1.pdfand http://sequence.aecom.yu.edu/chr12/Parm2.pdf) were tested.
Positive control primers were able to amplify fragments of the YACs and BACs while sarcospan primers were not (data not shown).
The size of circular YACs was estimated by melting the agarose plugs with yeast genomic DNA prior to CHEF analysis.