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Example sentences for: yac

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If the TAR vector recombines with a genomic DNA fragment, the URA3 gene is lost and the resulting YAC clone becomes resistant to 5-FOA.
YAC, yeast artificial chromosome
Although the coverage of the YAC and BAC physical maps was nearly complete, only direct sequencing of CFEOM1 patient DNA would yield definitive results about the status of the sarcospan gene in CFEOM1.
Probes were selected that flank known sites of genomic rearrangement based upon previous yeast artificial chromosome (YAC) and BAC/PAC mapping studies, together with the human sequence map available from the University of California at Santa Cruz (UCSC) Genome Browser [ 11 12 13 14 15 ] . Probes flanking the Prader-Willi/Angelman syndrome breakpoints, in addition to a characterized inv dup(15) rearrangement were selected to test the possibility that the pericentric inversion evolutionary breakpoint corresponded to a site of known human genomic instability (Figure 1).
Southern blot analysis of YAC DNA was performed with 32P labeled probes and the protocol described by Church and Gilbert [ 35 ] .