Words similar to xylene
Example sentences for: xylene
How can you use “xylene” in a sentence? Here are some example sentences to help you improve your vocabulary:
Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
Over the two-year process to eliminate methylene chloride from this operation, the adhesive manufacturer also eliminated methylene chloride from the adhesive by substituting aromatic hydrocarbons (xylene).
The specimens were sectioned at 5 micron in thickness, and used for immunohistochemical staining with ATM antibody as described [ 18 ] . Briefly, the sections were de-paraffinized in xylene solution, de-hydrated and rehydrated in 100%, 90% and 70% alcohol.
The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.
After dehydration in 95% ethanol twice for 1 min and in 100% ethanol for 1 min, sections were cleared in xylene for 2 min and were air-dried.