Words similar to xylene
Example sentences for: xylene
How can you use “xylene” in a sentence? Here are some example sentences to help you improve your vocabulary:
The sections were placed in 2% cobalt chloride solution for 3 minutes, washed in four 30-sec changes in 100 mM 2-amino-2-methyl-1-propanol at pH 9.4, developed in 1% ammonium sulfide solution for 3 minutes, washed in running tap water for 5 minutes, dehydrated in graded ethanol, cleared in xylene and mounted in Canada balsam.
After two 1-minute rinses in distilled water, the tissue sections were counterstained with hematoxilin, cleared and dehydrated by successive gradations through 70%, 95%, and 100% ethanol followed by a final passage in xylene.
After dehydration in 95% ethanol twice for 1 min and in 100% ethanol for 1 min, sections were cleared in xylene for 2 min and were air-dried.
Subsequently, the sections were rinsed 3 times in xylene and coverslipped using Permount (Fisher) as a mounting solution.
The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.
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