Words similar to xylene
Example sentences for: xylene
How can you use “xylene” in a sentence? Here are some example sentences to help you improve your vocabulary:
Paraffin-embedded sections were treated to remove the paraffin with xylene, prior to the ethanol treatment.
The sections were then counterstained and cleared in xylene.
Subsequently, the sections were rinsed 3 times in xylene and coverslipped using Permount (Fisher) as a mounting solution.
The amplified PCR products (20 μl total) were mixed with 10 μl of loading buffer (95% formamide, 10 mM EDTA, 0.1% xylene cyanol, 0.1% bromophenol blue) and 5 μl aliquots were applied to a 5% denaturing polyacrylamide gel.
Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
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