Words similar to xylene

Example sentences for: xylene

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Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
The specimens were sectioned at 5 micron in thickness, and used for immunohistochemical staining with ATM antibody as described [ 18 ] . Briefly, the sections were de-paraffinized in xylene solution, de-hydrated and rehydrated in 100%, 90% and 70% alcohol.
Reactions were stopped by addition of loading buffer: 80% formamide, 10 mM Na 2 EDTA (pH 8.0), and 1 mg/ml each bromophenol blue and xylene cyanol.
The slides were de-paraffinized with a total of three incubations in xylene, rehydrated through a graded alcohol series beginning with 100%, 95%, 70%, and 50% in PBS, and treated with 0.3% hydrogen peroxide in methanol to quench the endogenous peroxidase activity.
Subsequently, the sections were rinsed 3 times in xylene and coverslipped using Permount (Fisher) as a mounting solution.