Example sentences for: xyle

How can you use “xyle” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resistant bacteria would then be grown in a second medium or in the absence of a stress, and colonies scored for lack of promoter activity in the second condition by expression of the xylE reporter gene.

  • When the initial pool of kanamycin-resistant colonies sprayed with catechol, the colonies were light to dark yellow indicating a wide range of XylE activity.

  • Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.

  • For the screening step, we used the xylE gene because catechol 2,3 dioxygenase activity is easily measured by spraying colonies with catechol and the amount of color produced is a rough measure of promoter activity [ 10 ] . In our screen, white, pale yellow, and bright yellow colonies were observed, indicating a wide range of promoter activity.

  • In coinfection experiments, the ratio of M. smegmatis bacteria expressing the full-length Rv0365c ORF to xylE -expressing wild-type bacteria was 1:1 at time zero and increased to approximately 10:1 at 12 hours and to 11:1 at 24 hours (Figure 5).


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