Words similar to xyle
Example sentences for: xyle
How can you use “xyle” in a sentence? Here are some example sentences to help you improve your vocabulary:
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.
This indicates that the survival of the xylE -expressing strain was the same as that of the wild-type and could be used an internal reference by which to measure the survival of other clones.
These possibilities could be distinguished by transformation of plasmids from individual clones into the σ E-over-expressing strain and directly comparing of XylE activity in the presence and absence of σ E. Unfortunately, it appears that expression of XylE in M. smegmatis may be detrimental and may lead to plasmid instability and loss of the inserted DNA.
Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.
The following day, the plates were sprayed with 0.5 M catechol (Sigma) in 50 mM potassium phosphate (pH 7.5) to distinguish the xylE -expressing colonies (yellow) from the recombinant colonies (white).
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