Example sentences for: xyle

How can you use “xyle” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.

  • This indicates that the survival of the xylE -expressing strain was the same as that of the wild-type and could be used an internal reference by which to measure the survival of other clones.

  • These possibilities could be distinguished by transformation of plasmids from individual clones into the σ E-over-expressing strain and directly comparing of XylE activity in the presence and absence of σ E. Unfortunately, it appears that expression of XylE in M. smegmatis may be detrimental and may lead to plasmid instability and loss of the inserted DNA.

  • Replating of the yellow colonies produced a mixture of white and yellow colonies, suggesting that the expression of the xylE or aph gene in the SigE-expressing strain may be detrimental to the cell and may lead to plasmid instability.

  • The following day, the plates were sprayed with 0.5 M catechol (Sigma) in 50 mM potassium phosphate (pH 7.5) to distinguish the xylE -expressing colonies (yellow) from the recombinant colonies (white).


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