Words similar to xyle
Example sentences for: xyle
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This amplicon was Not I-digested and cloned into the Not I site of the pHIP1 plasmid containing the xylE gene to generate pHIP4.
In experiments in which THP-1 cells were infected at a MOI of 50:1 (results in ~1 phagocytosed M. smegmatis bacterium per macrophage) with a mixture containing equal numbers of bacteria of the xylE -expressing control strain and a strain carrying the cosmid vector pYUB178, the ratio of recovered white colonies to yellow colonies was 1:1 at all time points (data not shown).
For the screening step, we used the xylE gene because catechol 2,3 dioxygenase activity is easily measured by spraying colonies with catechol and the amount of color produced is a rough measure of promoter activity [ 10 ] . In our screen, white, pale yellow, and bright yellow colonies were observed, indicating a wide range of promoter activity.
In coinfection experiments with xylE -expressing bacteria, both sur2 and sur3 bacteria exhibited increased survival (Figure 3).
In this coinfection, the ratio of white (sur2) to yellow (xylE and Rv0365c expressing) colonies remained 1:1 through 12 hours and then increased to ~3:1 at 24 hrs, consistent with the above-described observations.