Words similar to xbai

Example sentences for: xbai

How can you use “xbai” in a sentence? Here are some example sentences to help you improve your vocabulary:

Stage V-VI oocytes were removed by partial laparotomy and defolliculated by collagenase treatment [ 11 ] . Cloned human Aquaporin1 DNA was provided by Dr. P. Agre [ 28 ] , linearized with XbaI and used as a template for in vitro synthesis of cRNA with T3 RNA polymerase.
The XbaI site used for insertion into the ROSA26 genomic locus [ 4] was replaced by a linker (PacI, SwaI, AscI), so that it could be digested with PacI and AscI, and receive the bigT sequence.
The PCR product was digested with SacI and XbaI and cloned into the SacI/XbaI sites of both pRS315 (pSW1264) and pRS316 (pSW1268) [ 66 ] . Plasmids with wild type SEC23 were constructed in the same manner using specific oligonucleotides (5' oligo sec23F5 with 5'-ATGAATATCTAGACCAGGGTGCC-3'; 3' oligo sec23-3 with 5'CCTTGGATCCGTAGTAAAGGCCACGCAG-3') and digestion of the PCR product with XbaI and BamHI to yield p SEC23/LEU2 (pSW1266) and p SEC23/URA3 (pSW1269).
A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].
To make pBigT, plasmid pSAβgeo was digested with ClaI and XbaI to remove the βgeo and bpA sequence but leave behind the SA, the ends were Klenow filled and the plasmid self-ligated.