Words similar to xbai
Example sentences for: xbai
How can you use “xbai” in a sentence? Here are some example sentences to help you improve your vocabulary:
A XbaI/NotI fragment from clone 4B1 and a NotI fragment from 6A1 hybridized to the probe and were subcloned in pBluesript II SK(-) (Stratagene) for further analysis.
Stage V-VI oocytes were removed by partial laparotomy and defolliculated by collagenase treatment [ 11 ] . Cloned human Aquaporin1 DNA was provided by Dr. P. Agre [ 28 ] , linearized with XbaI and used as a template for in vitro synthesis of cRNA with T3 RNA polymerase.
To generate pEGFP-COP1, pKS-COP1 was digested with ApaI and XbaI and the resulting fragment containing the MmCOP1 cDNA was subcloned into the same sites of the pEGFP I vector.
The PCR product was digested with SacI and XbaI and cloned into the SacI/XbaI sites of both pRS315 (pSW1264) and pRS316 (pSW1268) [ 66 ] . Plasmids with wild type SEC23 were constructed in the same manner using specific oligonucleotides (5' oligo sec23F5 with 5'-ATGAATATCTAGACCAGGGTGCC-3'; 3' oligo sec23-3 with 5'CCTTGGATCCGTAGTAAAGGCCACGCAG-3') and digestion of the PCR product with XbaI and BamHI to yield p SEC23/LEU2 (pSW1266) and p SEC23/URA3 (pSW1269).
A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].
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