Words similar to xba

Example sentences for: xba

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Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T
After confirmation by DNA sequencing, the Xba I- Xba I fragment was subcloned into pURA4.
Using the resulting plasmid pSB10 as template, the cloned product was PCR amplified with the vector forward and reverse primers, digested with Xba I and labeled as described above, resulting in Fragment B (see Figure 5).
To create p(NFAT) 3 -MLP-G-less a DNA fragment containing the three NFAT sites was recovered from the digestion of the p(NFAT) 3 -E1b-CAT with Xba I and ligated into the Xba I of pΔML200 (gift of J. Parvin) [ 32, 33].
p(NFAT) 3 -E1b-CAT, used as template DNA in the in vitro transcription assays, was created by inserting three direct copies of the murine IL-4 high affinity NFAT site (region -82 to -64 of the murine IL-4 promoter) into the Xba I site of plasmid E1b-CAT.