Words similar to xba
Example sentences for: xba
How can you use “xba” in a sentence? Here are some example sentences to help you improve your vocabulary:
p(NFAT) 3 -E1b-CAT, used as template DNA in the in vitro transcription assays, was created by inserting three direct copies of the murine IL-4 high affinity NFAT site (region -82 to -64 of the murine IL-4 promoter) into the Xba I site of plasmid E1b-CAT.
The PCR product was digested with Xba I and Mro I and ligated into pBS-KS +-NFATp that had been digested with Xba I and Mro I. pVL1392-HAX-NFATp(1-722) was made by subcloning a Nde I - Msc I fragment from pBS-KS +-NFATp(NdeI) into the Nde I and Sma I sites of pVL1392-HAX.
The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).
The CaCET1 gene was disrupted by insertion of a UAU1 cassette [ 16 ] . We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the Kpn I and Xba I sites of pBluescript KS +and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the Sac I and Sac II sites of pBluescript KS +The 3.8-kbp UAU1 gene was excised from pBME101 with Xba I and Sac II and inserted between the Xba I and Sac II sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1.
The control experiment ( Xba I digestion before the run) was done in a CHEF-DR III system (Bio-Rad) under the following conditions: electrophoresis buffer, 0.5 × TBE; 6 V/cm; angle, 120°; pulse time, 4 × 50 sec; run time, 20 hrs; temperature, 14°C.
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