Example sentences for: xba

How can you use “xba” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A 900 bp Xho1/Xba1 fragment of Hnf3α genomic DNA that included exon 1 and a portion of intron 1 was used as the 5' arm of homology.

  • The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).

  • Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T

  • To create p(NFAT) 3 -MLP-G-less a DNA fragment containing the three NFAT sites was recovered from the digestion of the p(NFAT) 3 -E1b-CAT with Xba I and ligated into the Xba I of pΔML200 (gift of J. Parvin) [ 32, 33].

  • The plasmid pMP8NEBΔLacZ was generated by ligating an 8 kb Xba1 fragment from pMP8 into the Xba1 site of pNEB193 (New England Biolabs) from which LacZ sequences had been removed as a NarI/EcoRI fragment [ 15 ] . This


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