Words similar to xba

Example sentences for: xba

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Replacement of the endogenous rec12 +locus with the rec12-Y98F allele was achieved by transformation and a pop-in, pop-out approach [ 61 63 ] . A 1.2 kbp Xba I- Xba I fragment of rec12 +was subcloned into pBluescript SKII(+) and subject to oligonucleotide directed mutagenesis [ 64 ] to simultaneously introduce a silent mutation generating an Eco RV site and to mutate the Y98 codon (GAG ATA TTT ATT ACA → GA G ATA T
The PCR product was digested with the restriction enzymes Xba I and Spe I and cloned into Xba I-digested pGEM-IIZf (Promega).
The control experiment ( Xba I digestion before the run) was done in a CHEF-DR III system (Bio-Rad) under the following conditions: electrophoresis buffer, 0.5 × TBE; 6 V/cm; angle, 120°; pulse time, 4 × 50 sec; run time, 20 hrs; temperature, 14°C.
After confirmation by DNA sequencing, the Xba I- Xba I fragment was subcloned into pURA4.
A 900 bp Xho1/Xba1 fragment of Hnf3α genomic DNA that included exon 1 and a portion of intron 1 was used as the 5' arm of homology.