Words similar to xasp

Example sentences for: xasp

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The assembly of myosin II monomers into filaments is regulated by phosphorylation of its heavy chains at three threonine residues at the C-terminus of the tail [ 9 10 ] . Dephosphorylation of these threonines is a prerequisite of filament assembly, as confirmed by the phenotypes of a 3xAsp mutant, in which the three threonines are replaced by three aspartate residues (mimicking the phosphorylated state) [ 11 ] . In vitro the 3xAsp myosin II is severely impaired for filament assembly, and in vivo 3xAsp myosin II fails to assemble or localize to the cortical cytoskeleton.
As this MHC mutation inhibits filament assembly [ 11 ] , the inability of GFP-3xASP myosin to form such structures suggests that these TIRF-resolved structures are discrete myosin II filaments.
This dimension is consistent with myosin II thick filaments measured from the in vitro electron microscopy photographs [ 29 ] . Using TIRF microscopy, these structures, putative filaments, are not apparent in GFP-3xAsp myosin II cells in which the three potential Thr phosphorylation targets have been replaced with Asp residues (data not shown).