Words similar to xala

Example sentences for: xala

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In contrast, cells expressing a non-phosphorylatable myosin II construct (3xAla myosin cells) display severe myosin overassembly into the cytoskeleton [ 11 ] , and excessive myosin localization to the cleavage furrow during cytokinesis [ 7 ] . The 3xAla myosin cells also display severe defects in chemotactic cell migration, demonstrating the importance of proper myosin II assembly dynamics in this process [ 12 ] .
The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail [ 24 ] . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.