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A comprehensive report mapping MHC sequences with X-ray crystal structures and relative binding strength is available.
The single crystal X-ray structure [ 14 ] and the 2-D NMR (CDCl 3 ) [ 6d ] of Taxotere likewise demonstrate hydrophobic association, in this case between the tert -butyl and the C2 phenyl group.
This hypothesis predicts that females with autoimmunity may be particularly prone to this mechanism of `inadequate tolerization' by virtue of extremely skewed X-chromosome inactivation.
Coverslips were rinsed with TBS containing 0.1% Triton X-100 (TBS-Tx) and blocked in TBS-Tx with 2% BSA (AbDil) for 45 minutes.
Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).