Words similar to x-
Example sentences for: x-
How can you use “x-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Hpa II/polymerase chain reaction assay for X-chromosome inactivation
Western blots were performed as described previously [ 13 ] . For all other antibodies, rat intercostal muscle was excised and homogenized in 20 mM HEPES, pH 7.4, with 20 mM Nad, 5 mM EDTA/EGTA, 0.5% Triton X-100, ImM DTT and protease inhibitors on ice.
They were then lysed in a protein kinase lysis buffer [50 mM HEPES, pH 7.5, 150 mM NaCI, 1.5 mM MgCI2, 1 mM EGTA, 1 mM sodium vanadate, 50 mM sodium fluoride, 20 mM beta-glycerophosphate, 10% (vol/vol) glycerol, 1% (vol/vol) Triton X-100, and protease inhibitors (1 mM PMSF, 1 ug/ml pepstatin A1, 1 ug/ml leupeptin, and 1 ug/ml aprotinin].
Quick extraction with 0.2% Triton X-100 in MTSB (30 seconds, 2 times), followed by fixation with 1% glutaraldehyde also preserved microextensions well indicating that fragmentation or removal of microextensions by extraction with 0.2% Triton X-100 does not occur within 1 min.
While this may be true for some proteins, the postulated cytoskeletal association of threonine558-phosphorylated moesin was maintained only when platelets were extracted with DOTMAC, but not with Triton X-100 [ 19].