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5% SDS at 55°C for 30 min, and twice with 0.1X SSPE at room temperature for 10 min.
This type of plot has been popular for microarray data, with the x - and y -axes corresponding to the mean intensity of the signals and their fold ratio, respectively, up to scaling constants [ 18 19 ] . For tag counts, the axes become the sum of the log counts and the fold change, respectively, with no rescaling necessary.
The protein:DNA complexes were resolved by electrophoresis through 5% nondenaturing polyacrylamide gels in 0.25 X TBE buffer.
During exposures, the arrays were kept moist with 2x SSC saturated filter paper wrapped in plastic wrap.
MoeB, as represented by the functionally characterized Ec-MoeB, consists of an N-terminal ThiF domain followed by a 2X CXXC domain with both CXXC pairs intact and no rhodanese-like domain.
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