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Furthermore, we observed some overlap in the families representing the L1-TDs 5_7396, 7_12643, and X_11447.
In this manner subpopulations of IIb and IId/x fibers were identified.
We discard probes that are saturated in both X and Y, or probe pairs such that PM-MM < 3 or PM < 3 in both X or Y. Here, corresponds to the standard deviation of the fluctuations in the background intensity.
5, 0.5 % Triton X 100) and resuspended in Kinase Buffer - 20 mM Tris-HCl pH 7.5, 5 mM MgCl 2 and 5 mM MnCl 2 (all from Sigma-Aldrich, Inc.).
The number of unique genes is as high as 40% for X. fastidiosa [ 25], and it is also very high for some other species [ 26].