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This could be either from increased Collagen X mRNA transcription within prehypertrophic and hypertrophic cells or from ectopic activation of Collagen X transcription in proliferating chondrocytes.
To each sample, an equal volume of 2X Microspotting solution (Cat.
This phenomenon occurs at a very early embryonic stage [ 9], and thus all females are mosaic and may occasionally exhibit extreme skewing towards one or the other parental X chromosome.
FISH was performed using whole chromosome probes to the X and 13 (Oncor, Gaithersburg, MD).
In contrast, we were unable to detect Bcl-2, Bax or Bcl-X L in adherent vascular lung neutrophils even after endotoxin administration (data not shown).