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First, defective tolerance from skewed X inactivation should only be directed at X-linked antigens that are polymorphic, and for which the individual is heterozygous.
Specific details on the AIM-9X, F-22, and ATIRCM/CMWS program experiences follow.
For example, in an average that considers 5 consecutive points, 5 consecutive values from the original series x(t) are each multiplied by 1, the results are added, then divided by 5 to produce the corresponding y(t).
Fixed embryos were washed for 30 minutes in 0.3% Triton-X 100 in PBS (PBT), and then blocked for 30 minutes with 2% Normal Goat Serum (NGS)/0.
FISH was performed using whole chromosome probes to the X and 13 (Oncor, Gaithersburg, MD).