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5-μl PCR reaction, with 0.2 mM dNTP, 1.5 mM MgCl 2 , 5 μl 10X PCR Buffer (Invitrogen, Carlsbad, CA, USA), and 1 μg Namalwa genomic DNA.
We detected two regions of suggestive linkage, one on chromosome 19 and the other on the X chromosome in a sample of Finnish ASPs.
The mitochondrial pellets were incubated with 1% osmium tetroxide (Electron Microscopy Sciences) in cacodylate buffer for one hour on ice, followed by rinsing 3 x 4 min in ice-cold double-distilled water.
However, tumors from Wnt-1 TG, Pten +/+ mice have low apoptotic rates (40 ± 16 per digital camera field using a 20x objective) and high proliferation rates (53% ± 11).
On the other hand, when oocytes were exposed to these same MG-132 doses from one h after initiating the culture until 6 h later when the chemical was washed out (4X) and cultured for an additional 17 h in media without MG-132, oocytes exposed to 25.