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Example sentences for: wtpai-

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wtPAI-1 lost its inhibitory activity at the same pace as the fibrosarcoma PAI-1, indicating that its functional half life as an inhibitor and its ability to be stabilized by vitronectin are similar to those of the fibrosarcoma PAI-1.
4A, the specificity of wtPAI-1, Glu351Ala and Glu351Arg after denatured by 6 M Gdn-HCl and dialyzed against 50 mM sodium acetate, pH 5.6, was 84 ± 15, 112 ± 18 and 68 ± 9 U/ug, respectively.
[ 17 ] Briefly, serially diluted wtPAI-1, or mutants were incubated with equal volume of uPA (50 μl, 50 U/ml) for 30 min at 37°C, followed by addition of 100 μl, 0.5 mM S-2444.
The specific inhibitory activities of wtPAI-1, Glu351Ala and Glu351Arg were assessed with uPA and S-2444.
The PAI-1 samples were incubated in the absence or in the presence of a two molar excess of vitronectin (the fibrosarcoma PAI-1 and the wtPAI-1 were incubated with vitronectin) at 37°C.