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Example sentences for: wtpai-

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Fibrosarcoma PAI-1, wtPAI-1 or its mutants were denatured with 6 M guanidine-hydrochloride (Gdn-HCl) (45 min at 37°C), followed by dialysis (4-16 hours at 4°C) against 50 mM sodium acetate, pH 5.6, containing 0.5 M NaCl and 0.01% Tween-20.
Expression and purification of wtPAI-1 and its mutants
It should be noted that all these mutants could be activated to a similar extent by 6 M Gnd-HCl (~20% inhibitory activity of the wtPAI-1, activated under the same conditions).
For the functional stability, wtPAI-1, or mutants were incubated at 37°C.
wtPAI-1 and mutants were incubated with four molar excess of thrombin (40 nM) in the absence or presence of vitronectin (30 nM).