Words similar to wtpai-

Example sentences for: wtpai-

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5 ug of Fibrosarcoma PAI-1, or wtPAI-1 was denatured by boiling for 5 min in a buffer consisted of 0.5% SDS, 50 mM β-mercaptoethanol and 50 mM Tris-HCl, pH 7.4.
The rate constants of tPA for wtPAI-1, Glu351Ala and Glu351Arg were similar (Table 4), however, The rate constants of inhibition toward uPA by Glu351Ala and Glu351Arg were 4 and 7-fold higher than by wtPAI-1, respectively.
wtPAI-1 lost its inhibitory activity at the same pace as the fibrosarcoma PAI-1, indicating that its functional half life as an inhibitor and its ability to be stabilized by vitronectin are similar to those of the fibrosarcoma PAI-1.
Complex formation of the fibrosarcoma PAI-1, wtPAI-1 or its mutants with single-chain tPA or uPA were performed as described earlier [ 20 ] . Samples of PAI-1s (5 U) were incubated in the absence or in the presence of a 2-fold excess of uPA or tPA in the activity assay buffer.
Interestingly, there was no significant difference between the mutants tested and the wtPAI-1 in their ability to inactivate either tPA or uPA (Table 3).