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Example sentences for: wtpai-

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The inhibitory activities of fibrosarcoma PAI-1, wtPAI-1, and its mutants were measured by a direct chromogenic assay using uPA (100 U/ug) and its substrate S-2444 in a microtiter plate as described earlier [ 20 ] . Briefly, fibrosarcoma PAI-1, wtPAI-1, or its mutants were diluted 1:10 in the activity measuring assay buffer (50 uM Tris-HCl, pH 7.5, containing 0.15 M NaCl and 100 ug/ml BSA).
These results show that the wtPAI-1 could inhibit tPA or uPA at the same rate as the fibrosarcoma PAI-1.
The molecular weight of wtPAI-1 was found to be lower than native PAI-1 (~50 kDa, Fig.
Characterization of wtPAI-1
Both mutants inhibited thrombin more efficiently than wtPAI-1, more importantly, Glu351Ala was better than Glu351Arg in inhibiting thrombin (Fig.