Words similar to wtpai-

Example sentences for: wtpai-

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1 μg/ml) was incubated at 37°C for 30 min with same amount of wtPAI-1, E351A and E351R in term of inhibitory activity toward uPA (20 U in the concentration range of 4-10 μg/ml) in absence or presence of vitronectin (60 μg/ml).
Although they had lower specific activities, all of the mutants mentioned above could still form SDS-stable complexes with tPA or uPA just as the wtPAI-1 (data not shown).
It should be noted that all these mutants could be activated to a similar extent by 6 M Gnd-HCl (~20% inhibitory activity of the wtPAI-1, activated under the same conditions).
Fibrosarcoma PAI-1, wtPAI-1 or its mutants were denatured with 6 M guanidine-hydrochloride (Gdn-HCl) (45 min at 37°C), followed by dialysis (4-16 hours at 4°C) against 50 mM sodium acetate, pH 5.6, containing 0.5 M NaCl and 0.01% Tween-20.
After activation with 6 M Gnd-HCl, each of the six mutants exhibited a relatively high inhibitory activity, but their specific activities were still much lower than that of wtPAI-1 (Fig.