Example sentences for: wt-α

How can you use “wt-α” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Hence the signaling mechanism, like that reported in normal myotubes and dysgenic myotubes expressing wt-α 1S , was Ca 2+entry independent [ 29 ] . We also expressed fs-α 1S in cultured myotubes from two available gene knock-out (KO) mice, lacking the endogenous β1a isoform of the skeletal muscle DHPR [ 30 ] and lacking RyR1 [ 31 ] . As shown in Fig.

  • We found that fs-α 1S and α 1 SΔ1-700 together expressed Ca 2+transients with a ΔF/Fo max similar to wt-α 1S control (not shown).

  • pSG5 wt-α 1S

  • Cells were fixed and processed for immunofluorescence as described [ 4 20 ] . The N-terminal fragment expressed by fs-α 1S or wt-α 1S was identified with a mouse monoclonal antibody against a T7 epitope fused to the N-terminus of α 1S . The anti-T7 antibody (Novagen, WI) was used at a dilution of 1:1000.

  • 6B) shows that the signal generated by fs-α 1S was significantly smaller than that generated by the control construct (wt-α 1S vs. fs-α 1S t-test significance p = 0.014) and smaller than that generated by the two coexpressed fragments (fs-α 1S vs. fs-α 1S M701I + α 1S Δ1-700 t-test significance p = 0.013).


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