Words similar to wortmannin
Example sentences for: wortmannin
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Treatment of Jurkat cells with Wortmannin had no effect on Rlk association with RAFTs (Fig.
We found that wortmannin and rapamycin had no effect on basal SP-A mRNA levels, at any concentration tested.
Two inhibitors of PI 3-kinase, wortmannin and LY294002, and an inhibitor of p70 S6 kinase activation, rapamycin, blocked the inhibitory effect of insulin on SP-A gene expression.
Insulin transduces its cellular signal in most cell types via two signaling pathways, i.e., the p44/42 mitogen-activated protein kinase (MAPK) pathway and the phosphatidylinositol 3-kinase (PI 3-kinase) pathway [ 8 ] . Insulin initially binds to the insulin receptor causing autophosphorylation of the β-subunit of the receptor [ 8 ] . The protein kinase domain of the β-subunit then phosphorylates tyrosine residues in insulin receptor substrate (IRS)-1, IRS-2, IRS-3, or Shc, which then activates the p44/42 MAPK signaling cascade [ 8 ] . PD98059 is a cell permeable, non-competitive, reversible inhibitor of MAPK/extracellular signal-regulated kinase kinase (MEK), a protein kinase upstream of p44/42 MAPK [ 9 ] . Tyrosine phosphorylated IRS also activates the PI 3-kinase pathway [ 8 10 ] . There are at least three, well-characterized inhibitors of the PI 3-kinase pathway, i.e., wortmannin, LY294002, and rapamycin [ 11 12 13 ] . Wortmannin and LY294002 block the activation of PI 3-kinase itself [ 11 12 ] , while rapamycin prevents the phosphorylation of a downstream effector in the pathway, p70 S6 kinase [ 13 ] .
LDH activity was not different from controls in cells treated for 12 to 16 hours with either 200 nM wortmannin, 5 μM LY294002, 20 nM rapamycin, or 10 μM PD98059 (data not shown).
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