Words similar to wortmannin
Example sentences for: wortmannin
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LDH activity was not different from controls in cells treated for 12 to 16 hours with either 200 nM wortmannin, 5 μM LY294002, 20 nM rapamycin, or 10 μM PD98059 (data not shown).
Wortmannin is a fungal metabolite that is a relatively selective inhibitor of PI 3-kinase with a half-maximal inhibition (IC 50 ) less than 5 nM [ 11 ] . In the wortmannin experiments, media were changed every 2 hours for a total of 12 hours in all of the conditions because wortmannin is unstable in solution at physiological pH [ 19 ] . The shorter incubation time with insulin in the wortmannin experiments resulted in a decreased inhibitory effect of insulin, i.e., an ~22% inhibition of SP-A mRNA levels (Fig.
2shows that wortmannin (100 nM) and rapamycin (50 nM) both abolished the insulin-mediated inhibition of SP-A gene expression.
H441 cells resemble bronchiolar epithelial Clara cells in phenotype and produce both SP-A and SP-B mRNA and protein [ 14 ] . The hormonal regulation of SP-A and SP-B gene expression in H441 cells is similar to that observed in differentiated type II cells in human fetal lung explants [ 6 7 15 16 ] . We treated H441 cells with the four inhibitors of signal transduction: wortmannin, LY294002, rapamycin and PD98059, to elucidate which signaling pathways are activated when insulin inhibits SP-A gene expression.
The cultured cells were subsequently exposed to fresh serum-free media and pretreated for 30-60 minutes with either control media that contained the vehicle for the inhibitors (dimethyl sulphoxide) or with media that contained wortmannin (5-200 nM), LY294002 (2-50 μM), rapamycin (1-100 nM), or PD98059 (2.
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