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Example sentences for: worthington

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Cells were dissociated in 50 ml tubes by enzymatic digestion with 0.25% Collagenase (Worthington Biochemical, Collagenase Type 2 - CLS 2) and 0.25% Trypsin/EDTA (Invitrogen/Gibco BRL) for 1 hour at 37°C, or until the soft tissues were floating in the solution.
Further, tissues were cut into smaller pieces and incubated at 37°C in Isocove's medium supplemented with 10% fetal calf serum and penicillin/streptomycin containing 400 U/ml Collagenase D (Boerrhinghem & Mannheim GmbH, Mannheim, Germany) and 25 U/ml DNAse (Worthington Biochemical, Lakewood, NJ) for 2-3 hours.
Briefly, the superior cervical ganglia were dissected from adult rats and incubated in Earle's balanced salt solution (Life Technologies, Rochelle, MD) containing 0.45 mg ml -1trypsin (Worthington, Freehold, NJ), 0.6 mg ml -1collagenase D (Boehringer Mannheim, Indianapolis, IN) and 0.05 mg ml -1DNAse I (Sigma, St. Louis, MO) for 1 hour at 35°C.