Words similar to whole-cell
Example sentences for: whole-cell
How can you use “whole-cell” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the whole-cell configuration, membrane currents in response to repeatedly applied voltage ramp pulses from -90 to -50 mV were recorded.
A549 cells or NHBEs were treated with CSC as described above and whole-cell protein extracts were prepared by scraping cells into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 μg/ml each of leupeptin and pepstatin, and 1 μM PMSF.
Lysates were clarified by centrifugation at 10,000 × g for 30 min at 4°C and the protein concentration of the whole-cell extracts was determined by the bicinchoninic protein assay (Pierce Biochemical Co., Rockford, IL, USA).
3Ashows the results of a western blot of whole-cell extracts from NHBEs exposed to increasing concentrations of CSC and probed for phosphorylated ERK1/2, the activated form of ERK1/2.
Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
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