Example sentences for: whole-cell

How can you use “whole-cell” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • NHBEs were treated with increasing concentrations of CSC and whole-cell extracts were immunoblotted for phosphorylated IκB.

  • Whole-cell recordings were performed as described previously [ 22 ] using an Axopatch 200B amplifier (Axon Instruments, Foster City, CA).

  • A549 cells or NHBEs were treated with CSC as described above and whole-cell protein extracts were prepared by scraping cells into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 μg/ml each of leupeptin and pepstatin, and 1 μM PMSF.

  • Lysates were clarified by centrifugation at 10,000 × g for 30 min at 4°C and the protein concentration of the whole-cell extracts was determined by the bicinchoninic protein assay (Pierce Biochemical Co., Rockford, IL, USA).

  • The nystatin-perforation method [ 37 ] was used to measure the membrane potential, and the standard method was used to measure whole-cell currents [ 38 ] . The resistance of the electrode, filled with the pipette solution, ranged from 2 to 4 MΩ.


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