Words similar to washes
Example sentences for: washes
How can you use “washes” in a sentence? Here are some example sentences to help you improve your vocabulary:
After PB washes, bound antibody was detected using 3,3'-diaminobenzidine (DAB, Sigma, St Louis, MO) as the chromogen.
2 ml for association/dissociation studies and 0.75 ml in saturation and competition experiments) were then transferred to the pre-coated 96-well filtration plates (Millipore fiberglass FB filters), filtered and washed over a vacuum manifold with six 300 μl per well washes of binding buffer supplemented with 2% hydoxypropyl cyclodextrin (HPCD; Sigma; ST Louis, USA) + 0.1% BSA to decrease non-specific binding.
Washington, PA) and buffered with PBS (or PBS with 0.2% Tween-20, PBST) for 10 minutes followed by 3 washes in PBS/PBST for 10 minutes each.
Rabbit polyclonal antibody against RSV-P protein has been described [ 21 ] . Indirect immunofluorescence and nuclear staining with DAPI was performed essentially as described previously [ 33 ] . A549 cells in monolayer, grown on cover slips, were washed in PBS and fixed in ice-cold 10% trichloracetic acid for 15 min, followed by successive washes in cold 70%, 90% and absolute ethanol for 3 min each.
After two PBS washes cells were resuspended in PBS and incubated with the primary antibody.
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