Example sentences for: washed

How can you use “washed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After overnight incubation, membranes were washed and incubated with peroxidase labeled secondary antibody (goat anti-mouse IgG and IgM, pre-absorbed with human serum and diluted 1: 2000; Jackson Immunoresearch, West Grove, PA) for 1 hour at room temperature.

  • The FNA material was immediately resuspended in ice-cold Iscove's medium without serum supplementation, and subsequently washed in PBS before RNA isolation.

  • After centrifugation, the cultures were washed once with 0.5 ml DMEM, treated with 0.5 ml serum-free media containing 0.1 units/ml penicillin and 0.1 μg/ml streptomycin, and returned to the incubator.

  • Filters were washed twice for 1 h (each) at 65°C in Church and Gilbert buffer.

  • The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).


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