Words similar to washed
Example sentences for: washed
How can you use “washed” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cells were washed with a 20-fold volume of MACS buffer, spun, and resus-pended in 500 μl buffer.
Subsequently, washed slides were incubated with streptavidin-peroxidase conjugate for 15 minutes, washed again, and incubated 5 minutes in substrate-chromogen solution, rinsed with PBS, washed in distilled water, counterstained 2 minutes with Harris hematoxylin diluted 1:20, dehydrated, and mounted.
Pooled Macroprep Q fractions containing GFP-BLM were loaded onto a 5 ml HiTrap metal chelate column (Amersham Pharmacia) charged with NiS0 4 equilibrated in Buffer B. The metal chelate resin was washed with successive 10 column volumes of Buffer B + 10mm imidazole, Buffer B + 50 mM imidazole, and Buffer B + l00 mM imidazole.
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
The boredom of jail is alleviated if one puts some puff (a.k.a. weed a.k.a. draw ) marijuana in with some burn (a.k.a. snout ) tobacco to make a joint marijuana cigarette, which, when washed down with some hooch illegally brewed alcohol can make the time pass a little quicker.