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Figure 6demonstrates HPB-ALL spreading on VCAM-1 over a period of 1 hour, where images from the first 10 minutes were obtained from recorded videotape, followed by a 60 minute time point.
Mock transfection on VCAM Mock transfected HPB-ALL cells have normal motility on immobilized VCAM-1 substrate.
TK-1 were seen to interact with SVEC through ICAM-1 as well MAdCAM-1, but not VCAM-1, since an antibody against MAdCAM-1 reduced adhesion to 27 ± 4.6% of maximum and an anti-ICAM-1 reduced adhesion to 54.
MAdCAM-1 (mucosal addressin cell adhesion molecule-1), IL-10 (Interleukin-10), HEV (high endothelial venular), ECAMs (endothelial cell adhesion molecules), IBD (inflammatory bowel diseases), UC (ulcerative colitis), CD (Crohn's disease), ICAM-1 (intracellular adhesion molecule 1), VCAM-1 (vascular adhesion molecule 1), NF-kB (nuclear transfection factor kB), TNF-α (tumor necrosis factor alpha), GM-CSF (granulocyte-macrophage colony-stimulating factor), GCSF (granulocute colony stimulating factor), ECL (enhanced chemiluminescence)
In the setting of IBD, the expression of ECAMs like ICAM-1, VCAM-1, and MAdCAM-1 (mucosal addressin cell adhesion molecule-1) is observed in experimental models of colitis, [ 2 3 4 5 ] and also within the inflamed human colon in Crohn's disease and ulcerative colitis [ 6 7 ] .