Words similar to unstimulated
Example sentences for: unstimulated
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Most significantly, a mixture of a lysate from unstimulated BCL 1 .3B3 cells harbouring wildtype Syk with a lysate from unstimulated DT40 expressing the K395A, 3F mutant Syk (see below), at 1:5, 1:1 and 5:1 ratios, showed no activity in the immune complex kinase assay (data not shown).
Therefore, we tested our anti-phospho-moesin antibodies with this method and, as shown in Figure 4H, the fluorescence intensity was too low to distinguish characteristic structures probably because the amount of phosphorylated moesin is low in unstimulated NIH3T3 cells (cf.
The average of the standard errors relative to the mean expression values (SE/mean signal intensity)*100% was 22% for untreated and 15% for IL-2-stimulated PBMCs, and 16% and 19% for the unstimulated and IL-2-treated purified T cell samples, respectively.
In untreated cells, no Rel A (solid bars) was detectable whereas in resting cells constitutive levels of p50 were observed (open bars), suggesting that unstimulated A549 epithelial cells contain p50 homodimers, which with regard to transcriptional activity have been shown to be functionally inert or inhibitory.
In CD8 +T cells from African HIV +patients, stimulated with env peptide, both IFN-γ and TNF-α production were increased over controls [ 52 ] . PBMC from the elderly (almost healthy donors) showed upregulation of TNF-α in unstimulated cultures [ 52 ] . HLA-DR activation of CD8 +CD57 +cells from elderly subjects strongly produced TNF-α [ 48 ] . Lipopolysaccharide-activation of T cells from aged donors induced higher levels of TNF-α than controls in two separate studies [ 53 31 ] . Finally, in HIV +patients receiving HIV type 1 antigen, both INF-γ and TNF-α were significantly increased in memory T cells activated by antigen [ 54 ] .
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