Example sentences for: unlabeled

How can you use “unlabeled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The concentration of unoccupied hERα was determined by ligand binding analyses as described previously [ 22 27 28 29 30 ] . Briefly, cytosols were incubated with 5 nM [ 3H] E 2 in the absence (total binding) or presence (nonspecific binding) of a 1000-fold molar excess of unlabeled DES for 16-20 hours at 4°C.

  • Binding was also abolished in the presence of excess unlabeled NF-κB, demonstrating the specificity of the probe.

  • Unlabeled PGE 2 , PGD 2 , and PGF 2α were purchased from Cayman Chemical (Ann Arbor).

  • Biotinylated CTP and UTP (Enzo Biochem Inc., NY) were used together with unlabeled dNTPs in the reaction, and unincorporated nucleotides were removed with RNeasy columns.

  • Binding was carried out in 250 μl volume of 50 mM Tris HCl buffer, pH 7.4 in the presence of about 0.5-1 nM [ 3H]-DAMGO and increasing concentrations (24-32) of unlabeled ligand.


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