Words similar to unlabeled
Example sentences for: unlabeled
How can you use “unlabeled” in a sentence? Here are some example sentences to help you improve your vocabulary:
The concentration of unoccupied hERα was determined by ligand binding analyses as described previously [ 22 27 28 29 30 ] . Briefly, cytosols were incubated with 5 nM [ 3H] E 2 in the absence (total binding) or presence (nonspecific binding) of a 1000-fold molar excess of unlabeled DES for 16-20 hours at 4°C.
Binding was also abolished in the presence of excess unlabeled NF-κB, demonstrating the specificity of the probe.
Unlabeled PGE 2 , PGD 2 , and PGF 2α were purchased from Cayman Chemical (Ann Arbor).
Biotinylated CTP and UTP (Enzo Biochem Inc., NY) were used together with unlabeled dNTPs in the reaction, and unincorporated nucleotides were removed with RNeasy columns.
Binding was carried out in 250 μl volume of 50 mM Tris HCl buffer, pH 7.4 in the presence of about 0.5-1 nM [ 3H]-DAMGO and increasing concentrations (24-32) of unlabeled ligand.
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