Words similar to uniset
Example sentences for: uniset
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Kidney and lymphoma cRNA samples containing either no modified NTPs or 1:1 DAP:A were hybridized in duplicate to Human Uniset I arrays; average kidney to lymphoma ratios were calculated and plotted (Figure 5) after removing signal outliers defined as a two-fold difference between replicates.
The intensities of these probe sets was measured after different array hybridization times to generate the data shown in Figure 3. Lastly, the probes on the Human Uniset I arrays which were designed to measure the endogenous human transcripts in polyA+ RNA were measured after different array hybridization times and with different amounts of labeled cRNA to generate the data shown in Figure 4A.
The data shown in Figures 2through 4were generated using the Codelink™ Human Uniset I arrays.
Differential expression ratios demonstrate very good correlation between the unmodified control sample and the 1:1 DAP:A sample (r = 0.95) on the Human Uniset I microarray.
Two μg of fragmented target cRNA was used for hybridization of each UniSet Human I Expression Bioarray (Motorola Life Sciences) containing 9589 probes (representing 9,203 unique accession numbers (genes), corresponding to approximately 8,935 unique clusters and 386 control probes, selected initially from GenBank Unigene build #125) or for hybridization to a microarray containing probes corresponding to 1100 human genes, each spotted 6 times per array.