Words similar to uninduced
Example sentences for: uninduced
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The vector pRL-TK (Promega), expressing the Renilla luciferase enzyme from the HSV thymidine kinase (TK) promoter, was included as an uninduced constitutively expressed internal control.
For uninduced cells, only endogenous PP1α RNA can be found associated with polysomes (Figure 11, second lane).
Western blotting for additional isoforms of PP1 reveals that this reduction in PP1α following induction is isotype-specific; when 6His-HA-PP1α levels increase, concomitant with a decrease in PP1α levels, PP1δ and PP1γ1 levels remain relatively unchanged from those found in uninduced cell lysate (Figure 10).
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).
However, in this instance there was some background growth of uninduced bacteria, consistent with the higher level of background splicing activity in vitro . If theophylline is regulating intron splicing in vivo , then the extent of bacterial growth should be dependent upon the concentration of theophylline introduced into the media (Figure 4c).