Words similar to uninduced
Example sentences for: uninduced
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For uninduced cells, only endogenous PP1α RNA can be found associated with polysomes (Figure 11, second lane).
1a) out of 17 showed good responsiveness (15 fold) and low uninduced background expression levels (0.
Western blotting for additional isoforms of PP1 reveals that this reduction in PP1α following induction is isotype-specific; when 6His-HA-PP1α levels increase, concomitant with a decrease in PP1α levels, PP1δ and PP1γ1 levels remain relatively unchanged from those found in uninduced cell lysate (Figure 10).
As shown in Figure 3, there is a predominant nuclear localization of the hemagglutinin epitope (panel A) compared to the low-level cytoplasmic and nuclear background reactivity seen in the uninduced LLWO2F control cells (panel B).
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).