Words similar to uninduced
Example sentences for: uninduced
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First, immunocomplexed 6His-HA-PP1α was tested for its ability to dephosphorylate phosphorylase a . Phosphorylated on a single serine residue, this substrate is often used to monitor the biochemical activity of PP1 in vitro [ 28 ] . As shown in Figure 5, greater phosphatase activity is detected in immunoprecipitates from induced LLWO2F cell lysates compared to uninduced.
Indeed, the abundance of endogenous PP1α RNA found in uninduced cells appears equal to or greater than the combined abundance of endogenous and 6His-HA-PP1α RNA (Figure 9), supporting the notion that some level of translational control may also exist.
However, in this instance there was some background growth of uninduced bacteria, consistent with the higher level of background splicing activity in vitro . If theophylline is regulating intron splicing in vivo , then the extent of bacterial growth should be dependent upon the concentration of theophylline introduced into the media (Figure 4c).
To inhibit any endogenous PP2A activity, which can also dephosphorylate phosphorylase a and thus interfere with PP1 activity measurements, these assays were carried out in the presence of 5 nM okadaic acid, which does not inhibit PP1 activity [ 30 ] . Although reproducible, we observed only modest increases in PP1-specific activity found in lysates from induced cells compared to the uninduced controls (Figure 7).
As shown in Figure 3, there is a predominant nuclear localization of the hemagglutinin epitope (panel A) compared to the low-level cytoplasmic and nuclear background reactivity seen in the uninduced LLWO2F control cells (panel B).
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