Words similar to unincorporated
Example sentences for: unincorporated
How can you use “unincorporated” in a sentence? Here are some example sentences to help you improve your vocabulary:
But when you have paid it all, the New York City Department of Taxation and Finance taps you on a figurative shoulder, reminds you that the city also has an Unincorporated Business Tax, and states firmly that free-lance writers gotta pay this too.
First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).
GGPG was iodinated with NaI or Na 125I (Pierce, Rockford, IL) by the iodobead method [ 41 42 ] . Unincorporated NaI or Na 125I was removed by solid phase extraction on a Sep-Pak C-18 Plus Cartridge.
Unincorporated dye and other contaminants were removed with SEQueaky Kleen 96 well kit (BIO-RAD Laboratories, Hercules, Ca.)
U0126 or vehicle was added to each well and the cells were then incubated for 15 min at 37°C in 5% CO 2 . Cells were washed to remove unincorporated [ 3H]-AA and then incubated in HHBSS supplemented with 0.05% BSA with either U0126 or vehicle.