Example sentences for: unincorporated

How can you use “unincorporated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • U0126 or vehicle was added to each well and the cells were then incubated for 15 min at 37°C in 5% CO 2 . Cells were washed to remove unincorporated [ 3H]-AA and then incubated in HHBSS supplemented with 0.05% BSA with either U0126 or vehicle.

  • The cells were then washed twice with ice-cold HBSS, spun at 250 g for 8 min to remove unincorporated radioactivity and resuspended in HBSS.

  • Coupling reactions were quenched by the addition of 41 μl of 0.1 M sodium acetate pH 5.2, and unincorporated dye was removed using QIAquick columns.

  • Unincorporated nucleotides were removed by size-exclusion chromatography on RNase-free Sephadex G-50 (Sigma) spin columns.

  • Labeled cells were incubated in 1 ml HEPES-buffered saline solution (HBS, composition in mM: NaCl, 145; KCl, 5; MgSO 4 , 1; CaCl 2 , 1.8; glucose, 10; HEPES, 10; pH 7.4) at 37°C for 30 min twice to wash out the unincorporated label.


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