Words similar to ung

Example sentences for: ung

How can you use “ung” in a sentence? Here are some example sentences to help you improve your vocabulary:

Standard reaction volume was 10 μl and contained 1X QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA), 0.7 μl of cDNA template and 100-500 nM of oligonucleotide primers.
The PCR mix per reaction included 10 μl SYBR Green buffer, 1.5 mM MgCl 2 , 1 μM dNTP mix (with dUTP), 0.5 μl CMV primer mix, 2.5 units Ampli Taq gold, 0.05 units of uracil-N-glycosylase (UNG, Epicentre, Madison, WI) that had been diluted 1:10 in UNG buffer, and molecular grade water to bring the volume up to 45 μl with the addition of 5 μl of cDNA or standard.
The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.
Initial steps of RT-PCR were 2 minutes at 50°C for UNG erase activation, followed by a 15 minute hold at 95°C.
The PCR consisted of 2 pre-incubation steps, 37°C/15 minutes and 95°C/10 minutes (for UNG activation and UNG denaturation/ Taq activation).