Words similar to unamplified
Example sentences for: unamplified
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At amounts greater or equal to 300 ng, the correlation coefficients between amplified and unamplified samples and among amplified samples remain about the same.
[ 24 ] . Their results were based on nine microarray experiments and showed concordance between amplified and unamplified samples, verifying four expressed and two differentially expressed genes using Northern and Western blotting and immunohistochemical assay.
However, when the unamplified poly(A) +RNA arrays performed weeks apart from different print batches were compared, the average correlation coefficient dropped to 0.89 ± 0.01, indicating that experimental variations due to differences in microarray printing, poly(A) +RNA isolation, and RNA labelling and hybridization contribute a small but detectable change in results.
Although one group has used unamplified total RNA extracted from ~2 × 10 4microdissected cells for hybridization on 5000 clone membrane-based arrays [ 15 ] , most groups perform RNA amplification for this purpose [ 16 17 18 ] , especially when using high-density slide-based arrays.
The gene expression of amplified total RNA from melanoma cell lines hybridized to 2000 gene microarrays was compared to that of unamplified total or poly(A) +RNA using cluster analysis and determining the number of outlier genes between single experiments.