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Example sentences for: ucsd
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We used regions of amino acid sequence conserved between the midasin genes in yeast and Schizosaccharomyces pombe to make a rough map of the gene onto the August 2001 freeze of the assembled human genome with the public domain Blat server at http://genome.ucsd.edu/index.html [ 54 55 ] . The resultant partial nucleotide sequence identified about four-fifths of the exons and was used to design the requisite PCR primers to verify all exon-intron boundaries in the gene by physical sequencing of appropriate PCR-amplified cDNA fragments.
Breast tissues were obtained from surgical specimens at the John Radcliffe Hospital, Oxford, UK and at the UCSD Medical Center.
The histological grade was assigned according to the modified Bloom-Richardson grading scheme [ 17 ] . Tissue samples were obtained and processed under approval of the UCSD Institutional Review Board for studying existing tissues (#011246X).
ERK was found to be constitutively phosphorylated in our study using MDCK cells from ATCC, in contrast to what has been reported previously for MDCK-D 1 , a subclone of MDCK selected for adrenergic receptor expression [ 39 40 ] . However, a side-by-side comparison of MDCK cells from ATCC and the MDCK-D 1 subclone (kindly provided by Dr. Paul Insel, UCSD) demonstrated constitutive activation of ERKs when both are grown at low density (not shown).
Subjects were recruited from a general outpatient pediatric clinic at the Perlman Ambulatory Center of the UCSD Medical Center, La Jolla, during July and August of 2000.
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