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Example sentences for: ucsd
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Subjects were recruited from a general outpatient pediatric clinic at the Perlman Ambulatory Center of the UCSD Medical Center, La Jolla, during July and August of 2000.
Housing and all procedures using nude mice were approved by the animal care committee at UCSD.
The wild-type IGF-IR cDNA or a mutant cDNA, which contained a methionine substitution for lysine 1003 in the ATP-binding site of the kinase domain, were cloned into the retroviral vector pLPONL [ 57 ] . The pseudo-typed viruses were generated and purified by the UCSD Viral Vector Core.
The histological grade was assigned according to the modified Bloom-Richardson grading scheme [ 17 ] . Tissue samples were obtained and processed under approval of the UCSD Institutional Review Board for studying existing tissues (#011246X).
ERK was found to be constitutively phosphorylated in our study using MDCK cells from ATCC, in contrast to what has been reported previously for MDCK-D 1 , a subclone of MDCK selected for adrenergic receptor expression [ 39 40 ] . However, a side-by-side comparison of MDCK cells from ATCC and the MDCK-D 1 subclone (kindly provided by Dr. Paul Insel, UCSD) demonstrated constitutive activation of ERKs when both are grown at low density (not shown).
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