Words similar to tyrp-

Example sentences for: tyrp-

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Monoclonal antibodies to OPN, to TYRP-1 and to TSP-1 were purchased commercially (OPN [1:100], Chemicon International, Inc., Temecula, CA, USA; TYRP-1 [1:80, clone G-17], Santa Cruz Biotechnology, Inc., CA, USA; TSP-1 [1:320], BD Transduction Laboratories, CA, USA).
We measured the expression levels of the OPN, TSP-1 and TYRP-1 genes using a quantitative PCR assay (RT-qPCR).
The sequences of the PCR primer pairs used for each gene are as follows: TYRP-1, 5'-GATGGCAGAGATGATCGGGA-3' and 5'-AGAAATTGCCGTTGCAGTGAC-3' ; TSP-1, 5'-CAGCAGCCGCTTTTATGT-3' and 5'-CCGAGTATCCCT-GAGCCCTC-3' ; OPN, 5'-TTGCAGCCTTCTCAGCCAA-3' and 5'-GGAGGCAAAAGCAAATCACTG-3' ; and GAPDH, 5'-CCACCCATGGCAAATTCC-3' and 5'-GGGATTTCCATTGATGACA-3'.
This procedure allowed OPN, TSP-1 and TYRP-1 mRNA analysis in specific cells of interest in each case, eliminating the contribution from normal adjacent cells that could cause difficulties in interpretation.
TYRP-1 is a type I membrane protein expressed by melanomas and normal melanocytes that was originally characterized as an oxidase in the melanin biosynthetic pathway.