Words similar to two-step

Example sentences for: two-step

How can you use “two-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

A two-step detection method was used to identify TRAIL.
For initial screening of the PCR primers, we incorporated a dye-labelled nucleotide with a two-step PCR protocol.
We describe here a comprehensive two-step search for HGT candidates in M. incognita, M. javanica and M. hapla using EST data [ 22 28 29 ] . Genome-to-genome comparisons were made to discover patterns of presence and absence that would indicate horizontally acquired genes.
Kinase-inactive (K430E) Btk was generated by a two-step PCR mutagenesis strategy [ 42].
Specifically, we show for the first time that both AGE and FFA PA induce PTEC apoptosis through CD36-mediated activation of src kinase, p38 MAPK, and caspase 3. Because high glucose stimulates CD36 expression in human PTECs and because CD36 expression is increased in apoptotic tubular epithelial cells in human DNP, we propose a two-step metabolic hit model relevant for TED, a hallmark of progression of human DNP.