Words similar to two-step

Example sentences for: two-step

How can you use “two-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

We developed protocols for quantitative two-step reverse transcriptase PCR to establish the relative amounts of mRNA transcripts encoding each of the β-tubulin isotypes (classes I, II, III, IVa, IVb, V, and VI).
To confirm the phenotypes of plasmids recovered in this two-step procedure, plasmids were recovered from 18 white colonies and individually transformed into M. smegmatis LR222::SEint.
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
GB virus C (GBV-C) RNA was detected using a two-step nested PCR amplification reaction from whole plasma RNA.
This two-step procedure was found to reduce the influence of bin size on mode estimation.