Words similar to two-step

Example sentences for: two-step

How can you use “two-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

We developed protocols for quantitative two-step reverse transcriptase PCR to establish the relative amounts of mRNA transcripts encoding each of the β-tubulin isotypes (classes I, II, III, IVa, IVb, V, and VI).
For initial screening of the PCR primers, we incorporated a dye-labelled nucleotide with a two-step PCR protocol.
Prior to genotyping, the double stranded polonies were made single stranded by stripping away the non-acrydited strand in a two-step procedure [ 3 ] . The polony DNA was denatured by incubation in a formamide buffer (1x SSC, 70% formamide, 25% doubly deionized-water) at 70 C for 15 min.
We describe here a comprehensive two-step search for HGT candidates in M. incognita, M. javanica and M. hapla using EST data [ 22 28 29 ] . Genome-to-genome comparisons were made to discover patterns of presence and absence that would indicate horizontally acquired genes.
This two-step procedure was found to reduce the influence of bin size on mode estimation.