Example sentences for: two-step

How can you use “two-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To confirm the phenotypes of plasmids recovered in this two-step procedure, plasmids were recovered from 18 white colonies and individually transformed into M. smegmatis LR222::SEint.

  • Transcription factors mediating the stress response to the expressed foreign protein may be able to recognize promoters within the library and promote transcription of the reporter genes, thus producing the false positives in the two-step screen.

  • We propose to use a two-step bootstrap resampling procedure [ 20] to estimate the variance of the estimate of the parameter γ in the regression model.

  • The construct was produced by a two-step PCR reaction using fs α 1S as template.

  • For initial screening of the PCR primers, we incorporated a dye-labelled nucleotide with a two-step PCR protocol.


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