Words similar to two-step

Example sentences for: two-step

How can you use “two-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

Quantitative RT-PCR assays were performed on the ABI Prism 7900HT sequence-detection system using the Taqman Reverse Transcription Kit (Applied Biosystems) and QuantiTech SYBRGreen PCR Kit (Qiagen) using a two-step reaction with protocols supplied by manufacturers.
Specifically, we show that glucose stimulates CD36 cell surface expression in proximal tubular epithelial cells (PTECs), and increased CD36 renders PTECs susceptible to both AGE- and PA-induced PTEC apoptosis by mediating sequential activation of src kinase, proapoptotic p38 MAPK, and caspase 3. Based on these findings, we propose a new two-step metabolic hit model for TED in the progression of DNP.
Btk is thought to be activated upon BCR cross-linking by a two-step mechanism involving phosphatidylinositol (PI) 3-kinase and the Src family PTK Lyn (reviewed in references [ 4, 5, 6]).
Kinase-inactive (K430E) Btk was generated by a two-step PCR mutagenesis strategy [ 42].
To confirm the phenotypes of plasmids recovered in this two-step procedure, plasmids were recovered from 18 white colonies and individually transformed into M. smegmatis LR222::SEint.