Words similar to two-color
Example sentences for: two-color
How can you use “two-color” in a sentence? Here are some example sentences to help you improve your vocabulary:
An early study on DNA microarray hybridization found that hybridization was strongly dependent on the rate constants for DNA adsorption/desorption in the non-probe covered regions of the surface, the two-dimensional diffusion coefficient, and the size of probes and targets and also suggested that sparse probe coverage may provide results equal to or better than those obtained with a surface totally covered with DNA probes [ 19 ] . A theoretical analysis of the kinetics of DNA hybridization demonstrated that diffusion was important in determining the time of required to reach equilibrium and was proportional to the equilibrium binding constant and to the concentration of binding sites [ 20 ] . A recent theoretical and experimental analysis of competitive hybridization in a two-color system demonstrated the need for the hybridization kinetics of the two probes to be the same [ 21 ] . An elegant study on the use of hybridization kinetics to differentiate specific from non-specific binding was recently published [ 22 ] . This study found that the hybridization kinetics for specific and non-specific binding of labeled cRNA to surface-bound oligodeoxyribonucleotides were significantly different, with specific binding requiring longer to reach hybridization equilibrium than non-specific binding.
For example, locally weighted linear regression (lowess) normalizations have been used to correct the systematic dependence of the log 2 of the red/green expression ratios on hybridization intensity [ 28 ] . The finding that competitive hybridization in a two-color system requires the hybridization kinetics of the two targets to be the same [ 21 ] may help explain the need for such normalizations.
In the workflow above, an Array and one or more BioMaterials (for two-color microarrays this would be two Labeled-Extracts) are combined to create a PhysicalBioAssay.
Examples of some of the uses and technologies supported include: one- and two-color spotted arrays (cDNAs, PCR products, or oligonucleotides) on glass or nylon, in situ synthesized oligonucleotides (Affymetrix/photolithography, ink-jet), RNA abundance (gene expression, polysome profiling), and DNA abundance (array-CGH, chromatin immunoprecipitation, genotyping).
With a two-color fluorescent in situ hybridization, most cells that expressed the ATPase 6 gene were also found to express the SP-B gene, indicating that type II pneumocytes and Clara cells are the cell types responsible for the over-expression of the ATPase 6 gene in P. carinii infection.
Loading...